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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SYK Monoclonal antibody

SYK Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C4A12

Cat No : CL594-66721

Print datasheet

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

验证数据展示

  • FC experiment of K-562 using CL594-66721

    FC experiment of K-562 using CL594-66721

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SYK (CL594-66721, Clone:4C4A12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66721-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66721 targets SYK in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen SYK fusion protein Ag17654 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name spleen tyrosine kinase
Synonyms DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK
Calculated Molecular Weight 635 aa, 72 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001645
Gene Symbol SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43405
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL594 SYK antibody CL594-66721Download protocol
Standard Protocols
Click here to view our Standard Protocols