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CoraLite®594-conjugated TDP-43 (for IF/FC) Recombinant antibody

TDP-43 (for IF/FC) Recombinant Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

16A22

Cat No : CL594-80002

Print datasheet

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 (for IF/FC)

验证数据展示

  • IF Staining of mouse brain using CL594-80002

    IF Staining of mouse brain using CL594-80002

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

  • IF Staining of mouse brain using CL594-80002

    IF Staining of mouse brain using CL594-80002

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

  • IF Staining of mouse brain using CL594-80002

    IF Staining of mouse brain using CL594-80002

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-80002

    FC experiment of HeLa using CL594-80002

    1X10^6 HeLa cells were stained with 0.2 ug CoraLite®594 Anti-Human TDP-43 (for IF/FC) (CL594-80002, Clone: 16A22) (red), or 0.2 ug CoraLite®594 Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 80002-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-80002 targets TDP-43 (for IF/FC) in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen TDP-43 (for IF/FC) fusion protein Ag1231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 (for IF/FC)
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_2883647
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
IF protocol for CL594 TDP-43 (for IF/FC) antibody CL594-80002Download protocol
Standard Protocols
Click here to view our Standard Protocols