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CoraLite® Plus 488-conjugated TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B1B12

Cat No : CL488-66845

Print datasheet

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

验证数据展示

  • FC experiment of MCF-7 using CL488-66845

    FC experiment of MCF-7 using CL488-66845

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TDRKH (CL488-66845, Clone:2B1B12) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-66845

    IF Staining of HepG2 using CL488-66845

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 TDRKH antibody (CL488-66845, Clone: 2B1B12 ) at dilution of 1:800.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66845-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66845 targets TDRKH in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat, pig
Immunogen TDRKH fusion protein Ag4720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tudor and KH domain containing
Synonyms TDRD2, TDRKH, tudor and KH domain containing
Calculated Molecular Weight 606 aa, 67 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC032690
Gene Symbol TDRKH
Gene ID (NCBI) 11022
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2W6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TDRKH antibody CL488-66845Download protocol
FC protocol for CL Plus 488 TDRKH antibody CL488-66845Download protocol
Standard Protocols
Click here to view our Standard Protocols