• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated TNFAIP3 Monoclonal antibody

TNFAIP3 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A11G6

Cat No : CL488-66695

Print datasheet

Synonyms

A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20

验证数据展示

  • IF Staining of HeLa using CL488-66695

    IF Staining of HeLa using CL488-66695

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated TNFAIP3 antibody (CL488-66695, Clone: 3A11G6) at dilution of 1:200, F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

  • FC experiment of HeLa using CL488-66695

    FC experiment of HeLa using CL488-66695

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TNFAIP3 (CL488-66695, Clone:3A11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66695-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66695 targets TNFAIP3 in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Rat
Immunogen TNFAIP3 fusion protein Ag18150 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 3
Synonyms A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20
Calculated Molecular Weight 790 aa, 90 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2883369
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21580
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and is involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TNFAIP3 antibody CL488-66695Download protocol
FC protocol for CL Plus 488 TNFAIP3 antibody CL488-66695Download protocol
Standard Protocols
Click here to view our Standard Protocols