TREM2 Polyclonal antibody
TREM2 Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
FC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MKN-45 cells, THP-1 cells |
| Positive FC detected in | BV-2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27599-1-AP targets TREM2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | FC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | TREM2 fusion protein Ag26319 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | triggering receptor expressed on myeloid cells 2 |
| Synonyms | TREM 2, TREM2, Trem2a, Trem2b, Trem2c |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC032362 |
| Gene Symbol | TREM2 |
| Gene ID (NCBI) | 54209 |
| RRID | AB_2880921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TREM2 antibody 27599-1-AP | Download protocol |
| FC protocol for TREM2 antibody 27599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study. | ||
Sci Adv TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma | ||
Front Immunol Identification of TREM2-positive tumor-associated macrophages in esophageal squamous cell carcinoma: implication for poor prognosis and immunotherapy modulation | ||
Nat Commun Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease | ||
Brain Res TREM2 regulates BV2 microglia activation and influences corticosterone-induced neuroinflammation in depressive disorders | ||
Cell Death Dis TREM2 deficiency impairs the energy metabolism of Schwann cells and exacerbates peripheral neurological deficits |
