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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2F6D1

Cat No : CL750-66576

Print datasheet

Synonyms

C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55

验证数据展示

  • WB analysis using CL750-66576

    WB analysis using CL750-66576

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66576 (c-Cbl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, mouse brain tissue, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 66576-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.

Tested Applications WB
Tested Reactivity Human, mouse
Immunogen c-Cbl fusion protein Ag23033 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Synonyms C CBL, CBL, CBL2, Proto oncogene c Cbl, RING finger protein 55, RNF55
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC132733
Gene Symbol CBL
Gene ID (NCBI) 867
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22681
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 c-Cbl antibody CL750-66576Download protocol
Standard Protocols
Click here to view our Standard Protocols