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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human R-Spondin-1 protein

Activity

4-20 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1328

Print datasheet

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Validation Data Gallery

  • Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human R-Spondin-1 (HZ-1328) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model.

    Recombinant human R-Spondin-1 (HZ-1328) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model.

Technical Specifications

GeneID 284654
Species Human
Expression HEK293
Activity 4-20 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession Number2QMKA7
Molecular Mass 36-50 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

R-Spondin 1 is an approximately 40kDA, glycosylated protein that serves as a major agonist of the Wnt signaling pathway. It regulates the turnover of the LRP6 co-receptor by preventing its internalization by DKK-1. R-Spondin 1 plays several roles in embryonic development including sex phenotype reversal, female sex determination, and ovarian differentiation. It also promotes stem cell turnover in the mammary glands, intestine, colon, and kidneys. Increased R-Spondin 1 expression has been linked to growth and migration of ovarian cancer cells as well as decreased sensitivity of gliomas to radiation-based therapies (PMID: 17804805, 22439850, 32749219, 30572097).

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Publications

SpeciesTitle
mouse

Nat Commun

Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells

Authors - Yang Sun
View Article
human

Mol Neurobiol

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis

Authors - Yipeng Zhao
View Article