Animal-free Recombinant Human Pleiotrophin is expressed in Human 293 cells as a secreted unglycosylated monomer. PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid- induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development.
Pleiotrophin is a secreted growth factor that induces neurite outgrowth being mitogenic for fibroblasts, epithelial, and endothelial cells. It binds anaplastic lymphoma kinase (ALK) inducing MAPK pathway activation, as an important step in the anti-apoptotic signaling of PTN and cell proliferation (PMID:1768439; 1733956; 11278720)