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HER2/ErbB2 Polyclonal antibody
HER2/ErbB2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | 4T1 cells, SGC-7901 cells, MCF-7 cells, HeLa cells |
Positive IP detected in | SGC-7901 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18299-1-AP targets HER2/ErbB2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
Synonyms | ERBB2, CD340, HER-2, HER2, HER 2/neu |
Calculated Molecular Weight | 138 kDa |
Observed Molecular Weight | 185 kDa |
GenBank Accession Number | BC167147 |
Gene Symbol | HER2/ErbB2 |
Gene ID (NCBI) | 2064 |
RRID | AB_2099264 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04626 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
IHC protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
IF protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
IP protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Cell Res Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers. | ||
Small Efficient Delivery of GSDMD-N mRNA by Engineered Extracellular Vesicles Induces Pyroptosis for Enhanced Immunotherapy | ||
Nat Commun Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer. | ||
J Nanobiotechnology Accurate HER2 determination in breast cancer: a prominent COF-immobilized enzyme-enhanced electrochemical aptasensor employing 4-acetamidophenol as an efficient mediator |