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IDE Monoclonal antibody

IDE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E10G1

Cat No : 67106-1-Ig

Print datasheet

Synonyms

Abeta degrading protease, FLJ35968, IDE, INSULYSIN

验证数据展示

  • WB analysis using 67106-1-Ig

    WB analysis using 67106-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67106-1-Ig

    WB analysis of HeLa using 67106-1-Ig

    WB result of IDE antibody (67106-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, A431 cells, HEK-293 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67106-1-Ig targets IDE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen IDE fusion protein Ag17161 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Abeta degrading protease
Synonyms Abeta degrading protease, FLJ35968, IDE, INSULYSIN
Calculated Molecular Weight 1019 aa, 118 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC096336
Gene Symbol IDE
Gene ID (NCBI) 3416
RRIDAB_2882410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).

实验方案

Product Specific Protocols
WB protocol for IDE antibody 67106-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Nutr Food Res

Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway

Authors - Qian Su
View Article