HRP-conjugated GFP tag Antibody 0 Publications

Mouse Monoclonal| Catalog number: HRP-66002

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Species specificity:
recombinant protein, aequorea victoria

Positive WB detected in:
Recombinant protein

Recommended dilution:
WB : 1:5000-1:50000

Product Information


Source:
Mouse

Purification method:
Protein A Purified

Isotype:
IgG2a

Storage:
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Recombinant Protein

Full name:
GFP tag

Calculated molecular weight:
26 kDa

Observed molecular weight:
-

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


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