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验证数据展示

  • IF Staining of HepG2 using CL488-66422

    IF Staining of HepG2 using CL488-66422

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 PDI antibody (CL488-66422, Clone: 2E6A11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66422

    FC experiment of HeLa using CL488-66422

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PDI Monoclonal antibody (CL488-66422, Clone:2E6A11)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PDI Monoclonal antibody

PDI Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66422

Print datasheet

CloneNo. 2E6A11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66422-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66422 targets PDI in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
Calculated Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2883333
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDI antibody CL488-66422Download protocol
FC protocol for CL Plus 488 PDI antibody CL488-66422Download protocol
Standard Protocols
Click here to view our Standard Protocols