验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells |
| Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66422-1-Ig targets PDI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, bovine |
| Immunogen | PDI fusion protein Ag1747 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | prolyl 4-hydroxylase, beta polypeptide |
| Synonyms | P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC014504 |
| Gene Symbol | PDI |
| Gene ID (NCBI) | 5034 |
| RRID | AB_2881794 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDI antibody 66422-1-Ig | Download protocol |
| IHC protocol for PDI antibody 66422-1-Ig | Download protocol |
| IF protocol for PDI antibody 66422-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells | ||
Biochim Biophys Acta Mol Cell Res SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization | ||
J Cell Sci A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function | ||
Mol Pharm Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization. | ||
Cell Signal HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression. | ||
Biochim Biophys Acta Gen Subj Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels |
