SignalBright Plus Chemiluminescent Substrate

Product Information

SignalBright Plus is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Plus provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours. Signal intensity is up to 2X that of SignalBright Pro.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: Up to 24 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· X2 Signal intensity verses SignalBright Pro 

· Minimal optimization re quired

Instructions for Use

SignalBright Plus Detection Kit is prepared by combining a 1:1 ratio of Chemiluminescent Substrate Solution and  Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-0.5 µg/ml

· Recommended HRP-conjugated secondary antibody concentration: 2-20 ng/ml