二抗

Proteintech提供全面的、高灵敏度二抗产品,这些二抗经充分验证可用于检测未标记的一抗。

这些二抗分别与各种荧光染料和酶偶联,通过了蛋白质印迹、免疫荧光、免疫组织化学、流式细胞术和ELISA等免疫学相关应用的充分验证,可满足各种实验需求。
其中ChromoTek 的Nano-Secondaries® 是一种新型二抗,能够得到更高分辨率和更清晰的成像效果。这类纳米抗体/VHH能与一抗发生高亲和力的结合,并具备物种和亚型特异性。
CoraLite®是Proteintech公司自有的具有良好光稳定性、发光强度以及较宽pH适应性,可完美胜任科研实验要求的染料系列。

HRP标记

二抗名称 货号 应用
HRP-conjugated Streptavidin SA00001-0 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) SA00001-1 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Fragment Specific SA00001-1-A ELISA, WB
HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) SA00001-2 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Goat IgG(H+L) SA00001-3 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) SA00001-4 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Swine IgG(H+L) SA00001-5 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Chicken IgY (IgG)(H+L) SA00001-6 ELISA, WB
HRP-conjugated Anti-Heavy Chain of Rabbit IgG SA00001-7H ELISA, WB, IP
HRP-conjugated IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific SA00001-7L ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Mouse IgG(H+L) SA00001-8 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) SA00001-9 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Rat IgG(H+L) SA00001-10 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Human IgG(H+L) SA00001-11 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Guinea pig IgG(H+L) SA00001-12 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Horse IgG(H+L) SA00001-13 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Chicken IgY (IgG)(H+L) SA00001-14 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Rat IgG(H+L) SA00001-15 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Sheep IgG(H+L) SA00001-16 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Human IgG(H+L) SA00001-17 ELISA, WB
HRP -Protein A SA00001-18 WB
HRP-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain SA00001-19 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG,FcγSubclass 1 Specific SA00012-1 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG,FcγSubclass 2a Specific SA00012-2 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, FcγSubclass 2b Specific SA00012-3 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, FcγSubclass 2c Specific SA00012-4 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, FcγSubclass 3 Specific SA00012-5 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgM, μ Chain Specific SA00012-6 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgA (α) SA00012-7 ELISA, WB

纳米二抗

  • 一步法染色
  • 重组抗体
  • 免疫荧光分辨率更高

Nano-Secondaries相较于传统二抗具有一定优势.

  • Nano-Secondaries与一抗的预孵育或同时孵育,即一步孵育,减少孵育时间并增强多重检测能力。
  • 生产Nano-Secondaries的重组过程确保了批次间高度一致性。
  • 更小结构的Nano-Secondaries已被证明可以提高超分辨率显微镜中的图像分辨率。

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Featured Products

Recent Publications

Acta Biochim Biophys Sin (Shanghai)

Effect of Sox9 on TGF-β1-mediated atrial fibrosis

Hechuan Wang

Related

Secondary antibody selection

How to choose the best secondary antibody for your application

Non visible text
Spectra viewer

Design and plan your experiments using our spectra viewer

Non visible text
CoraLite® fluorescent dye-conjugated antibodies

CoraLite dyes have equivalent brightness to the Alexa Fluor® dyes.

Non visible text