二抗

Proteintech提供全面的、高灵敏度二抗产品,这些二抗经充分验证可用于检测未标记的一抗。

这些二抗分别与各种荧光染料和酶偶联,通过了蛋白质印迹、免疫荧光、免疫组织化学、流式细胞术和ELISA等免疫学相关应用的充分验证,可满足各种实验需求。
其中ChromoTek 的Nano-Secondaries® 是一种新型二抗,能够得到更高分辨率和更清晰的成像效果。这类纳米抗体/VHH能与一抗发生高亲和力的结合,并具备物种和亚型特异性。
CoraLite®是Proteintech公司自有的具有良好光稳定性、发光强度以及较宽pH适应性,可完美胜任科研实验要求的染料系列。

HRP标记

二抗名称 货号 应用
即用型HRP标记羊抗兔二抗 PR30011 IHC
即用型HRP标记羊抗小鼠二抗 PR30012 IHC
即用型HRP标记羊抗兔/小鼠二抗 PR30009 IHC
HRP-conjugated Streptavidin SA00001-0 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) SA00001-1 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Fragment Specific SA00001-1-A ELISA, WB
HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) SA00001-2 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Goat IgG(H+L) SA00001-3 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) SA00001-4 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Swine IgG(H+L) SA00001-5 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Chicken IgY (IgG)(H+L) SA00001-6 ELISA, WB
HRP-conjugated Mouse Anti-Heavy Chain of Rabbit IgG SA00001-7H ELISA, WB, IP
HRP-conjugated IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific SA00001-7L ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Mouse IgG(H+L) SA00001-8 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) SA00001-9 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Rat IgG(H+L) SA00001-10 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Human IgG(H+L) SA00001-11 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Guinea pig IgG(H+L) SA00001-12 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Horse IgG(H+L) SA00001-13 ELISA, WB
HRP-conjugated Affinipure Donkey Anti-Chicken IgY (IgG)(H+L) SA00001-14 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Rat IgG(H+L) SA00001-15 ELISA, WB
HRP-conjugated Affinipure Rabbit Anti-Sheep IgG(H+L) SA00001-16 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Human IgG(H+L) SA00001-17 ELISA, WB
HRP -Protein A SA00001-18 WB
HRP-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain SA00001-19 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific SA00012-1 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific SA00012-2 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific SA00012-3 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Subclass 2c Specific SA00012-4 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific SA00012-5 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgM, μ Chain Specific SA00012-6 ELISA, WB
HRP-conjugated Affinipure Goat Anti-Mouse IgA (α) SA00012-7 ELISA, WB

CoraLite® Plus 750标记

二抗名称 货号 应用
CoraLite Plus 750-conjugated Goat anti-rabbit IgG(H+L) SA00015-1 WB

纳米二抗

  • 一步法染色
  • 重组抗体
  • 免疫荧光分辨率更高

Nano-Secondaries相较于传统二抗具有一定优势.

  • Nano-Secondaries与一抗的预孵育或同时孵育,即一步孵育,减少孵育时间并增强多重检测能力。
  • 生产Nano-Secondaries的重组过程确保了批次间高度一致性。
  • 更小结构的Nano-Secondaries已被证明可以提高超分辨率显微镜中的图像分辨率。

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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