二抗

Proteintech提供全面的、高灵敏度二抗产品,这些二抗经充分验证可用于检测未标记的一抗。

这些二抗分别与各种荧光染料和酶偶联,可满足您的各种实验需求。这些产品的质量通过了蛋白质印迹、免疫荧光、免疫组织化学、流式细胞术和ELISA等免疫学相关应用的充分验证。
ChromoTek 的Nano-Secondaries® 是一种新型二抗,能够得到更高分辨率和更清晰的成像效果。这类纳米抗体/VHH能与一抗发生高亲和力的结合,并具备物种和亚型特异性。

纳米二抗

  • 一步法染色
  • 重组抗体
  • 免疫荧光分辨率更高

Nano-Secondaries相较于传统二抗具有一定优势.

  • Nano-Secondaries与一抗的预孵育或同时孵育,即一步孵育,减少孵育时间并增强多重检测能力。
  • 生产Nano-Secondaries的重组过程确保了批次间高度一致性。
  • 更小结构的Nano-Secondaries已被证明可以提高超分辨率显微镜中的图像分辨率。

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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