二抗
Proteintech提供全面的、高灵敏度二抗产品，这些二抗经充分验证可用于检测未标记的一抗。
这些二抗分别与各种荧光染料和酶偶联，通过了蛋白质印迹、免疫荧光、免疫组织化学、流式细胞术和ELISA等免疫学相关应用的充分验证，可满足各种实验需求。
其中ChromoTek 的Nano-Secondaries® 是一种新型二抗，能够得到更高分辨率和更清晰的成像效果。这类纳米抗体/VHH能与一抗发生高亲和力的结合，并具备物种和亚型特异性。
CoraLite®是Proteintech公司自有的具有良好光稳定性、发光强度以及较宽pH适应性，可完美胜任科研实验要求的染料系列。
HRP标记
AMCA标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| AMCA–conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00010-1 | IF, FC |
| AMCA–conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00010-2 | IF, FC |
| AMCA–conjugated Affinipure Donkey Anti-Goat IgG(H+L) | SA00010-3 | IF, FC |
| AMCA–conjugated Affinipure Rabbit Anti-Goat IgG(H+L) | SA00010-4 | IF, FC |
AP标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| Alkaline Phosphatase–conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00002-1 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00002-2 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Donkey Anti-Goat IgG(H+L) | SA00002-3 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Donkey Anti-Mouse IgG(H+L) | SA00002-4 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) | SA00002-5 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Donkey Anti-Rat IgG(H+L) | SA00002-6 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Donkey Anti-Human IgG(H+L) | SA00002-7 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Goat Anti-Human IgG(H+L) | SA00002-8 | ELISA, WB |
| Alkaline Phosphatase–conjugated Affinipure Goat Anti-Rat IgG(H+L) | SA00002-9 | ELISA, WB |
Biotin标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| Biotin-conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00004-1 | ELISA, WB, IHC/ICC |
| Biotin-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00004-2 | ELISA, WB, IHC/ICC |
| Biotin-conjugated Affinipure Donkey Anti-Goat IgG(H+L) | SA00004-3 | ELISA, WB, IHC/ICC |
| Biotin-SP-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) | SA00004-4 | ELISA, WB, IHC/ICC |
| Biotin-SP-conjugated Affinipure Goat Anti-Human IgG(H+L) | SA00004-6 | ELISA, WB, IHC/ICC |
| Biotin-SP-conjugated Affinipure Goat Anti-Rat IgG(H+L) | SA00004-8 | ELISA, WB, IHC/ICC |
| Biotin-SP-conjugated Affinipure Donkey Anti-Mouse IgG(H+L) | SA00004-9 | ELISA, WB, IHC/ICC |
| Biotin-conjugated Affinipure Donkey Anti-Human IgG(H+L) | SA00004-10 | ELISA, WB, IHC/ICC |
| Biotin-conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) | SA00004-11 | ELISA, WB, IHC/ICC |
CoraLite®488标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) | SA00013-1 | IF, FC |
| CoraLite488-conjugated Goat Anti-Rabbit IgG(H+L) | SA00013-2 | IF, FC |
| CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L) | SA00013-5 | IF, FC |
| CoraLite488-conjugated Donkey Anti-Rabbit IgG(H+L) | SA00013-6 | IF, FC |
| CoraLite488-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain | SA00014-3 | IF |
| CoraLite488-conjugated Mouse Anti-Heavy Chain of Rabbit IgG | SA00014-4 | IF, FC |
CoraLite®594标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| CoraLite594 – conjugated Goat Anti-Mouse IgG(H+L) | SA00013-3 | IF, FC |
| CoraLite594 – conjugated Goat Anti-Rabbit IgG(H+L) | SA00013-4 | IF, FC |
| CoraLite594 – conjugated Donkey Anti-Mouse IgG(H+L) | SA00013-7 | IF, FC |
| CoraLite594 – conjugated Donkey Anti-Rabbit IgG(H+L) | SA00013-8 | IF, FC |
| CoraLite594-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain | SA00014-2 | IF |
| CoraLite594-conjugated Mouse Anti-Heavy Chain of Rabbit IgG | SA00014-5 | IF, FC |
CoraLite®647标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| CoraLite647-conjugated Mouse Anti-Heavy Chain of Rabbit IgG | SA00014-6 | IF, FC |
| CoraLite647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) | SA00014-7 | IF, FC |
| CoraLite647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) | SA00014-8 | IF, FC |
| CoraLite647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) | SA00014-9 | IF, FC |
| CoraLite647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) | SA00014-10 | IF, FC |
CoraLite® Plus 750标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| CoraLite Plus 750-conjugated Goat anti-rabbit IgG(H+L) | SA00015-1 | WB |
Cy3标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| Cy3–conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00009-1 | IF, FC |
| Cy3–conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00009-2 | IF, FC |
| Cy3–conjugated Affinipure Donkey Anti-Goat IgG(H+L) | SA00009-3 | IF, FC |
| Cy3–conjugated Affinipure Rabbit Anti-Goat IgG(H+L) | SA00009-4 | IF, FC |
FITC标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| Fluorescein (FITC)–conjugated Affinipure Goat Anti-Mouse IgG(H+L) | SA00003-1 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Goat Anti-Rabbit IgG(H+L) | SA00003-2 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Donkey Anti-Goat IgG(H+L) | SA00003-3 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Rabbit Anti-Goat IgG(H+L) | SA00003-4 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) | SA00003-8 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Donkey Anti-Mouse IgG(H+L) | SA00003-9 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Goat Anti-Rat IgG(H+L) | SA00003-11 | IF, FC |
| Fluorescein (FITC)–conjugated Affinipure Goat Anti-Human IgG(H+L) | SA00003-12 | IF, FC, WB |
Rhodamine标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| Rhodamine (TRITC)–conjugated Goat Anti-Mouse IgG(H+L) | SA00007-1 | IF, FC |
| Rhodamine (TRITC)–conjugated Goat Anti-Rabbit IgG(H+L) | SA00007-2 | IF, FC |
| Rhodamine (TRITC)–conjugated Donkey Anti-Goat IgG(H+L) | SA00007-3 | IF, FC, WB |
| Rhodamine (TRITC)–conjugated Rabbit Anti-Goat IgG(H+L) | SA00007-4 | IF, FC |
| Rhodamine (TRITC)–conjugated Goat Anti-Rat IgG(H+L) | SA00007-7 | IF, FC |
| Rhodamine (TRITC)–conjugated Goat Anti-Human IgG(H+L) | SA00007-10 | IF, FC |
| Rhodamine (TRITC)–conjugated Donkey Anti-Human IgG(H+L) | SA00007-11 | IF, FC |
R-PE标记
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| R-PE–conjugated Goat Anti-Rabbit IgG(H+L) | SA00008-2 | IF, FC |
| R-PE–conjugated Donkey Anti-Goat IgG(H+L) | SA00008-3 | IF, FC |
| R-PE–conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG(H+L) | SA00008-9 | IF, FC |
Alexa Flour 488标记纳米二抗
Alexa Flour 568标记纳米二抗
Alexa Flour 647标记纳米二抗
重链特异性二抗
轻链特异性二抗
| 二抗名称 | 货号 | 应用 |
|---|---|---|
| HRP-conjugated IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific | SA00001-7L | ELISA, WB |
| HRP-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain | SA00001-19 | ELISA, WB |
| CoraLite488-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain | SA00014-3 | IF |
| CoraLite594-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain | SA00014-2 | IF |
纳米二抗
-
一步法染色
-
重组抗体
-
免疫荧光分辨率更高
Nano-Secondaries相较于传统二抗具有一定优势.
- Nano-Secondaries与一抗的预孵育或同时孵育，即一步孵育，减少孵育时间并增强多重检测能力。
- 生产Nano-Secondaries的重组过程确保了批次间高度一致性。
- 更小结构的Nano-Secondaries已被证明可以提高超分辨率显微镜中的图像分辨率。
Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
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3,647 Publications
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Affinipure Goat Anti-Rabbit IgG (H+L)
| Conjugate: | HRP |
| Applications: | ELISA, WB |
2,690 Publications
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Affinipure Goat Anti-Mouse IgG (H+L)
| Conjugate: | HRP |
| Applications: | ELISA, WB |
188 Publications
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Affinipure Goat Anti-Rabbit IgG (H+L)
| Conjugate: | CoraLite488 |
| Applications: | IF, Flow cyt |
281 Publications
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Affinipure Goat Anti-Rabbit IgG (H+L)
| Conjugate: | FITC |
| Applications: | IF, Flow cyt |
187 Publications
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Affinipure Goat Anti-Rabbit IgG (H+L)
| Conjugate: | Cy3 |
| Applications: | IF, Flow cyt |
207 Publications
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Affinipure Goat Anti-Mouse IgG (H+L)
| Conjugate: | FITC |
| Applications: | IF, Flow cyt |
See all secondary antibodies
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