标签和内参抗体

在科学研究中,内参蛋白往往不是主要研究对象,但是他们对研究的参照意义是不可或缺的,尤其是正确解释免疫印迹(WB)实验结果。

内参蛋白和标签蛋白的表达水平可为目标蛋白表达水平的变化提供一个参照,确保目标蛋白水平的任何变化都来自于样品的差异,而不是由于实验操作导致的。Proteintech提供包括GAPDH、Tubulin、Beta Actin等常用内参抗体和GST tag,6*His tag,GFP tag等标签抗体。

查看所有标签和内参抗体

Use our table below to help select a loading control for your sample type with a different molecular weight to your protein of interest.

内参抗体

适用范围 名称 分子量 抗体类型 货号 应用
胞浆或全细胞 GAPDH 36 kDa 鼠单抗 60004-1-Ig ChIP, CoIP, FC, IF, IHC, IP, WB
兔多抗 10494-1-AP CoIP, FC, IF, IHC, IP, RIP, WB
β-Actin 42 kDa 鼠单抗 66009-1-Ig ChIP, CoIP, FC, IF, IHC, IP, WB
兔多抗 20536-1-AP FC, IF, IHC, IP, WB
α-Tubulin 50-55 kDa 鼠单抗 66031-1-Ig FC, IF, IHC, IP, WB
兔多抗 11224-1-AP CoIP, FC, IF, IHC, IP, WB
兔多抗 14555-1-AP IF, IHC, WB
重组兔单抗 80762-1-RR IF, WB
β-Tubulin 50-55 kDa 鼠单抗 66240-1-Ig IF, IHC, IP, WB
兔多抗 10068-1-AP FC, IF, IHC, IP, WB
兔多抗 10094-1-AP CoIP, FC, IF, IHC, IP, WB
重组兔单抗 80713-1-RR FC, IF, IP, WB
Vinculin 117 kDa 鼠单抗 66305-1-Ig CoIP, FC, IF, IHC, WB
细胞核膜 Lamin A/C 65-75 kDa 兔多抗 10298-1-AP FC, IF, IP, WB
Lamin B1 66-72 kDa 鼠单抗 66095-1-Ig FC, IF, IHC, IP, WB
兔多抗 12987-1-AP ChIP, FC, IF, IHC, IP, WB
细胞核 TBP 33-43 kDa* 鼠单抗 66166-1-Ig FC, IF, IHC, IP, WB
兔多抗 22006-1-AP IF, IHC, IP, WB
PCNA 36 kDa 鼠单抗 60097-1-Ig FC, IF, IHC, IP, WB
兔多抗 10205-2-AP FC, IF, IHC, IP, WB
Histone-H3 15-17 kDa 兔多抗 17168-1-AP ChIP, FC, IF, IP, WB
YY1 65-70 kDa 鼠单抗 66281-1-Ig ChIP, CoIP, IF, IHC, IP, WB
兔多抗 22156-1-AP ChIP, IF, IHC, IP, WB
膜蛋白 ATP1A1 97-110 kDa 兔多抗 14418-1-AP CoIP, IF, IHC, IP, WB
兔多抗 55187-1-AP FC, IF, IHC, WB
线粒体 VDAC1/Porin 31-37 kDa 鼠单抗 66345-1-Ig IF, IHC, WB
兔多抗 10866-1-AP CoIP, IF, IHC, WB
COX4I1 17-20 kDa 鼠单抗 66110-1-Ig FC, IF, IHC, WB
兔多抗 11242-1-AP ChIP, FC, IF, IHC, IP, WB
COX4I2 17-20 kDa 兔多抗 11463-1-AP IF, IHC, IP, WB
细胞增殖 BrdU 鼠单抗 66241-1-Ig IF, IHC
全血/血浆/血清 Transferrin 77 kDa 鼠单抗 66171-1-Ig IF, IHC, WB
兔多抗 17435-1-AP IF, IHC, WB
Albumin 66 kDa 鼠单抗 66051-1-Ig IF, IHC, IP, WB
兔多抗 16475-1-AP CoIP, IF, IHC, IP, WB
*TBP在人类是37-43 kDa,在小鼠和大鼠中是33-36 kDa

标签抗体 

名称 抗体类型 货号 应用
6*His, His-tag 鼠单抗 66005-1-Ig ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB
兔多抗 10001-0-AP IF, IP, WB
Flag tag 鼠单抗 66008-4-Ig ChIP, CoIP, IP, WB, IF
兔多抗 20543-1-AP ChIP, CoIP, FC, IF, IHC, IP, RIP, WB
GFP tag 鼠单抗 66002-1-Ig ChIP, CoIP, IF, IHC, IP, WB, FC
兔多抗 50430-2-AP CoIP, IF, IHC, IP, IP-MS, RIP, WB
GST tag 鼠单抗 66001-2-Ig CoIP, IF, IP, WB
兔多抗 10000-0-AP ChIP, IF, IP, WB, FC
HA tag 鼠单抗 66006-2-Ig CoIP, FC, IF, IP, PLA, WB
兔多抗 51064-2-AP ChIP, CoIP, IF, IHC, IP, WB
MYC tag 鼠单抗 60003-2-Ig CoIP, IF, IP, WB
兔多抗 16286-1-AP CoIP, FC, IF, IHC, IP, WB
V5 tag 兔多抗 14440-1-AP IF, IP, WB
S1 tag 鼠单抗 66165-1-Ig WB
MBP tag 鼠单抗 66003-1-Ig CoIP, IF, IHC, IP, WB
兔多抗 15089-1-AP IF, IHC, WB

标记内参/标签抗体

类别 鼠单抗名称 货号 应用
HRP标记内参抗体 HRP-anti GAPDH HRP-60004 IF, IHC, WB
HRP-anti β-Actin HRP-66009 IP, WB
HRP-anti α-Tubulin HRP-66031 WB
HRP标记标签抗体 HRP-anti 6*His, His-tag HRP-66005 Blocking Assay, WB
HRP-anti GFP tag HRP-66002 IF, WB
HRP-anti GST tag HRP-66001 IP, WB
HRP-conjugated DYKDDDDK Tag HRP-66008 WB
荧光标记内参抗体 CoraLite594-Conjugated Beta Actin CL594-66009 FC (Intra), IF, WB
CoraLite488-Conjugated Beta Actin CL488-66009 FC, IF, WB
CoraLite®532-Conjugated Beta Actin CL532-66009 IF
CoraLite®555-Conjugated Beta Actin CL555-66009 FC (Intra), IF
CoraLite®647-Conjugated Beta Actin CL647-66009 FC
CoraLite594-Conjugated Alpha Tubulin CL594-66031 FC, IF, WB
CoraLite488-Conjugated Alpha Tubulin CL488-66031 FC (Intra), IF, WB
CoraLite®555-Conjugated Alpha Tubulin CL555-66031 FC (Intra), IF
CoraLite®647-Conjugated Alpha Tubulin CL647-66031 FC (Intra)
荧光标记标签抗体 CoraLite®488-conjugated 6*His, His-Tag CL488-66005 IF
CoraLite®594-conjugated 6*His, His-Tag CL594-66005 IF
CoraLite®647-conjugated 6*His, His-Tag CL647-66005 IF
FITC-conjugated DYKDDDDK tag FITC-66008 IF
CoraLite®488-conjugated DYKDDDDK tag CL488-80010 IF
CoraLite®594-conjugated DYKDDDDK tag CL594-80010 IF
CoraLite®647-conjugated DYKDDDDK tag CL647-80010 IF
CoraLite®488-conjugated GFP tag CL488-66002 WB
CoraLite®594-conjugated GFP tag CL594-66002 FC, IF, WB
CoraLite®594-conjugated GFP tag CL594-50430 IF
CoraLite®488-conjugated GST tag CL488-66001 WB
CoraLite®647-conjugated GST tag CL647-10000 FC
CoraLite®594-conjugated HA tag CL594-51064 IF
CoraLite®488-conjugated MYC tag CL488-60003 IF
CoraLite®647-conjugated MYC tag CL647-60003 FC (Intra), IF

同型对照抗体

名称 货号 应用
Mouse IgG1 isotype control 66360-1-Ig WB ,FC ,ELISA
Mouse IgG2a isotype control 66360-2-Ig WB ,FC ,ELISA
Mouse IgG2b isotype control 66360-3-Ig WB ,FC ,ELISA

Proteintech offers a variety of antibodies for the identification, immunoprecipitation, and purification of proteins with genetically engineered epitope tags.