标签和内参抗体
在科学研究中,内参蛋白往往不是主要研究对象,但是他们对研究的参照意义是不可或缺的,尤其是正确解释免疫印迹(WB)实验结果。
内参蛋白和标签蛋白的表达水平可为目标蛋白表达水平的变化提供一个参照,确保目标蛋白水平的任何变化都来自于样品的差异,而不是由于实验操作导致的。Proteintech提供包括GAPDH、Tubulin、Beta Actin等常用内参抗体和GST tag,6*His tag,GFP tag等标签抗体。
Use our table below to help select a loading control for your sample type with a different molecular weight to your protein of interest.
内参抗体
适用范围 | 名称 | 分子量 | 抗体类型 | 货号 | 应用 |
胞浆或全细胞 | GAPDH | 36 kDa | 鼠单抗 | 60004-1-Ig | WB, IF, FC, IP, IHC, ChIP, CoIP |
兔多抗 | 10494-1-AP | IHC, WB, IP, IF, FC, CoIP, RIP | |||
β-Actin | 42 kDa | 鼠单抗 | 66009-1-Ig | WB, IF, FC, IP, IHC, ChIP, CoIP | |
重组兔单抗 | 81115-1-RR | IF, WB | |||
兔多抗 | 20536-1-AP | FC, IF, IHC, WB, IP | |||
α-Tubulin | 50-55 kDa | 鼠单抗 | 66031-1-Ig | CoIP, IF, IHC, IP, WB, FC | |
重组兔单抗 | 80762-1-RR | IF, WB | |||
兔多抗 | 11224-1-AP | WB, IP, IHC, FC, IF, CoIP | |||
兔多抗 | 14555-1-AP | IF, IHC, WB,ELISA | |||
β-Tubulin | 50-55 kDa | 鼠单抗 | 66240-1-Ig | WB, IHC, IF, IP | |
重组兔单抗 | 80713-1-RR | FC, IF, IP, WB | |||
兔多抗 | 10094-1-AP | WB, IF, IP, IHC, FC, CoIP | |||
兔多抗 | 10063-2-AP | WB, IP, CoIP, ELISA | |||
Vinculin | 117 kDa | 鼠单抗 | 66305-1-Ig | WB, IF, IP, IHC, FC, CoIP | |
细胞核膜 | Lamin A/C | 65-75 kDa | 兔多抗 | 10298-1-AP | IP, FC, WB, IF, IHC |
Lamin B1 | 66-72 kDa | 鼠单抗 | 66095-1-Ig | FC, IF, IHC, IP, WB | |
兔多抗 | 12987-1-AP | IHC, IP, WB, FC, IF, ChIP | |||
细胞核 | TBP | 33-43 kDa* | 鼠单抗 | 66166-1-Ig | WB, IP, IHC, IF, FC |
兔多抗 | 22006-1-AP | WB, IP, IF, IHC | |||
PCNA | 36 kDa | 鼠单抗 | 60097-1-Ig | IHC, FC, IP, WB, IF | |
兔多抗 | 10205-2-AP | Cell Treatment, CoIP, IF, IHC, WB, FC, IP | |||
Histone-H3 | 15-17 kDa | 兔多抗 | 17168-1-AP | ChIP, CoIP, IF, IP, WB, IHC, FC | |
鼠单抗 | 68345-1-Ig | IF, IHC, IP, WB | |||
YY1 | 65-70 kDa | 鼠单抗 | 66281-1-Ig | WB, IF, IP, IHC, ChIP, CoIP | |
兔多抗 | 22156-1-AP | ChIP, IF, IHC, IP, WB, CoIP | |||
膜蛋白 | ATP1A1 | 97-110 kDa | 兔多抗 | 14418-1-AP | CoIP, IF, IHC, IP, WB |
兔多抗 | 55187-1-AP | WB, IHC, FC, IF | |||
线粒体 | VDAC1/2 | 31-37 kDa | 兔多抗 | 10866-1-AP | CoIP, IF, IHC, WB |
VDAC1/Porin | 31-37 kDa | 鼠单抗 | 66345-1-Ig | IF, IHC, IP, WB | |
兔多抗 | 55259-1-AP | CoIP, FC, IF, IHC, IP, PLA, WB | |||
COX4I1 | 17-20 kDa | 鼠单抗 | 66110-1-Ig | FC, IF, IHC, WB | |
兔多抗 | 11242-1-AP | FC, IF, IHC, IP, WB, ChIP | |||
细胞增殖 | BrdU | - | 鼠单抗 | 66241-1-Ig | FC, IF, IHC, WB |
全血/血浆/血清 | Transferrin | 77 kDa | 鼠单抗 | 66171-1-Ig | IHC, WB, IF |
兔多抗 | 17435-1-AP | WB, IHC, IF, IP | |||
Albumin | 66 kDa | 鼠单抗 | 66051-1-Ig | WB, IP, IHC, IF | |
兔多抗 | 16475-1-AP | CoIP, IF, IHC, WB, IP |
标签抗体
名称 | 抗体类型 | 货号 | 应用 |
6*His, His-tag | 鼠单抗 | 66005-1-Ig | Cell Treatment, ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB |
兔多抗 | 10001-0-AP | IF, IP, WB, IHC | |
DYKDDDDK tag | 鼠单抗 | 66008-4-Ig | ChIP, CoIP, IF, IP, RIP, WB |
重组兔单抗 | 80010-1-RR | CoIP, FC, IF, IP, WB | |
兔多抗 | 20543-1-AP | ChIP, CoIP, FC, IF, IHC, IP, RIP, WB | |
GFP tag | 鼠单抗 | 66002-1-Ig | ChIP, CoIP, IF, IHC, IP, WB, FC |
兔多抗 | 50430-2-AP | ChIP, CoIP, IF, IHC, IP, IP-MS, RIP, WB | |
GST tag | 鼠单抗 | 66001-2-Ig | CoIP, IF, IP, WB |
重组兔单抗 | 80006-1-RR | WB | |
兔多抗 | 10000-0-AP | ChIP, CoIP, IF, IP, WB, FC | |
HA tag | 鼠单抗 | 66006-2-Ig | ChIP, CoIP, ELISA, IF, IP, PLA, WB |
重组兔单抗 | 81290-1-RR | IF, WB | |
兔多抗 | 51064-2-AP | ChIP, CoIP, IF, IHC, IP, WB | |
MYC tag | 鼠单抗 | 60003-2-Ig | CoIP, IF, IP, WB |
兔多抗 | 16286-1-AP | CoIP, IF, IHC, IP, WB | |
MBP tag | 鼠单抗 | 66003-1-Ig | CoIP, IF, IHC, IP, WB |
兔多抗 | 15089-1-AP | CoIP, IF, IHC, WB | |
V5 tag | 兔多抗 | 14440-1-AP | IF, IP, WB |
S1 tag | 鼠单抗 | 66165-1-Ig | WB |
标记内参/标签抗体
类别 | 鼠单抗名称 | 货号 | 应用 |
HRP标记内参抗体 | HRP-anti GAPDH | HRP-60004 | IF, IHC, WB |
HRP-anti β-Actin | HRP-66009 | IP, WB | |
HRP-anti α-Tubulin | HRP-66031 | WB | |
HRP标记标签抗体 | HRP-anti 6*His, His-tag | HRP-66005 | Blocking Assay, WB |
HRP-anti GFP tag | HRP-66002 | IF, WB | |
HRP-anti GST tag | HRP-66001 | IP, WB | |
HRP-conjugated DYKDDDDK Tag | HRP-66008 | WB | |
荧光标记内参抗体 | CoraLite594-Conjugated Beta Actin | CL594-66009 | FC (Intra), IF, WB |
CoraLite488-Conjugated Beta Actin | CL488-66009 | FC, IF, WB | |
CoraLite®532-Conjugated Beta Actin | CL532-66009 | IF | |
CoraLite®555-Conjugated Beta Actin | CL555-66009 | FC (Intra), IF | |
CoraLite®647-Conjugated Beta Actin | CL647-66009 | FC | |
CoraLite594-Conjugated Alpha Tubulin | CL594-66031 | FC, IF, WB | |
CoraLite488-Conjugated Alpha Tubulin | CL488-66031 | FC (Intra), IF, WB | |
CoraLite®555-Conjugated Alpha Tubulin | CL555-66031 | FC (Intra), IF | |
CoraLite®647-Conjugated Alpha Tubulin | CL647-66031 | FC (Intra) | |
荧光标记标签抗体 | CoraLite®488-conjugated 6*His, His-Tag | CL488-66005 | IF |
CoraLite®594-conjugated 6*His, His-Tag | CL594-66005 | IF | |
CoraLite®647-conjugated 6*His, His-Tag | CL647-66005 | IF | |
FITC-conjugated DYKDDDDK tag | FITC-66008 | IF | |
CoraLite®488-conjugated DYKDDDDK tag | CL488-80010 | IF | |
CoraLite®594-conjugated DYKDDDDK tag | CL594-80010 | IF | |
CoraLite®647-conjugated DYKDDDDK tag | CL647-80010 | IF | |
CoraLite®488-conjugated GFP tag | CL488-66002 | WB | |
CoraLite®594-conjugated GFP tag | CL594-66002 | FC, IF, WB | |
CoraLite®594-conjugated GFP tag | CL594-50430 | IF | |
CoraLite®488-conjugated GST tag | CL488-66001 | WB | |
CoraLite®647-conjugated GST tag | CL647-10000 | FC | |
CoraLite®594-conjugated HA tag | CL594-51064 | IF | |
CoraLite®488-conjugated MYC tag | CL488-60003 | IF | |
CoraLite®647-conjugated MYC tag | CL647-60003 | FC (Intra), IF |
同型对照抗体
名称 | 货号 | 应用 |
Mouse IgG1 isotype control | 66360-1-Ig | WB ,FC ,ELISA |
Mouse IgG2a isotype control | 66360-2-Ig | WB ,FC ,ELISA |
Mouse IgG2b isotype control | 66360-3-Ig | WB ,FC ,ELISA |
Proteintech offers a variety of antibodies for the identification, immunoprecipitation, and purification of proteins with genetically engineered epitope tags.
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