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CoraLite®488-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC, IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey

Applications

FC, IF, WB

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D4H5

Cat No : CL488-66009

Print datasheet

Synonyms

ACTB, actin, actin, beta, Beta Actin, Cell and organelle markers, Cytoskeleton Marker

验证数据展示

  • WB analysis using CL488-66009

    WB analysis using CL488-66009

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66009 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using CL488-66009

    IF Staining of MDCK using CL488-66009

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66009

    FC experiment of HeLa using CL488-66009

    1X10^6 HeLa cells were stained with 0.20ug beta Actin antibody (CL488-66009, red) and control antibody (blue). Fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IF detected inMDCK cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66009-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of CL488-66009 is Beta Actin Fusion Protein expressed in E. coli.

Tested Applications FC, IF, WB
Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name actin, beta
Calculated molecular weight 42 kDa
GenBank accession numberNM_001101
Gene symbol ACTB
UniprotP60709
Gene ID (NCBI) 60
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.

实验方案

Product Specific Protocols
WB protocol for CL488 Beta Actin antibody CL488-66009Download protocol
IF protocol for CL488 Beta Actin antibody CL488-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols