CoraLite®488-conjugated Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for FC, IF, WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, hamster, zebrafish, monkey
Applications
FC, IF, WB
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
2D4H5
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells |
Positive IF detected in | MDCK cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
The immunogen of CL488-66009 is Beta Actin Fusion Protein expressed in E. coli.
Tested Applications | FC, IF, WB |
Tested Reactivity | human, mouse, rat, hamster, zebrafish, monkey |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Fusion Protein |
Full Name | actin, beta |
Calculated molecular weight | 42 kDa |
GenBank accession number | NM_001101 |
Gene symbol | ACTB |
Uniprot | P60709 | Gene ID (NCBI) | 60 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.
实验方案
Product Specific Protocols | |
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WB protocol for CL488 Beta Actin antibody CL488-66009 | Download protocol |
IF protocol for CL488 Beta Actin antibody CL488-66009 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |