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Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey, dog and More (9)

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2D4H5

Cat No : 66009-1-Ig

Print datasheet

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, K-562 cells, HHSC-T6 cells, NIH/3T3 cells, 4T1 cells, CHO cells, mouse pancreas, rat brain, rat pancreas
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 66009-1-Ig is Beta Actin Fusion Protein expressed in E. coli.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications ChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey, dog
Cited ReactivityA.fumigatus strains, Aedes albopictus, Arabidopsis, bovine, canine, chicken, Cyprinus carpio, dog, Drosophila, duck
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name actin, beta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_001101
Gene symbol ACTB
UniprotP60709
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



实验方案

Product Specific Protocols
WB protocol for Beta Actin antibody 66009-1-IgDownload protocol
IHC protocol for Beta Actin antibody 66009-1-IgDownload protocol
IF protocol for Beta Actin antibody 66009-1-IgDownload protocol
IP protocol for Beta Actin antibody 66009-1-IgDownload protocol
FC protocol for Beta Actin antibody 66009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Cell-autonomous adiposity through increased cell surface GLUT4 due to ankyrin-B deficiency.

Authors - Damaris N Lorenzo
ratWB

Mol Med Rep

Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.

Authors - Qian Wang
ratWB

J Cell Mol Med

Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.

Authors - Yi Guan
humanWB

Onco Targets Ther

miR-222-3p promotes osteosarcoma cell migration and invasion through targeting TIMP3.

Authors - Jianping Guo

Mol Omics

Gene expression profile-based drug screen identifies SAHA as a novel treatment for NAFLD.

Authors - Zhujun Cheng
mouseWB

Front Pharmacol

Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet.

Authors - Jing-Hua Peng