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Beta Actin Monoclonal antibody 2107 Publications

Mouse Monoclonal| Catalog number: 66009-1-Ig |CloneNo.: 2D4H5

Featured Product KD/KO validated

浓度: 427 μg/ml

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Related Primary Antibodies

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
CoIP, IF, IHC, WB


Images See all

WB analysis of multi-cells using 66009-1-Ig

Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).

IF Staining of MDCK using 66009-1-Ig

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB analysis using 66009-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB analysis of A549 cells using 66009-1-Ig

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

IP experiment of HeLa using 66009-1-Ig

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

FC experiment of HeLa using 66009-1-Ig

1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

IHC staining of human kidney using 66009-1-Ig

Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).

Species Specificity:
human, mouse, rat, hamster, zebrafish, monkey, dog
Cited Species:
Aedes albopictus, Arabidopsis, bovine, canine, chicken, Cyprinus carpio, dog, Drosophila, duck, fish
Positive WB detected in:
multi-cells, Jurkat cells, A549 cells, CHO cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:500-1:2000

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
actin, beta
Calculated molecular weight:
42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.

Protocols

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