验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 87 publications below |
产品信息
HRP-66009 targets Beta Actin in WB applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey samples.
| 经测试应用 | WB Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, hamster, zebrafish, monkey |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actin, beta |
| 别名 | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | NM_001101 |
| 基因名称 | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2883836 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P60709 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Beta Actin antibody HRP-66009 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm | ||
Nat Commun Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice | ||
Theranostics Platelets camouflaged nanovehicle improved bladder cancer immunotherapy by triggering pyroptosis | ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. | ||
Bone Res Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration. |