• Featured Product
  • KD/KO Validated

HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey

Applications

WB and More (1)

Conjugate

HRP

CloneNo.

2D4H5

Cat No : HRP-66009

Print datasheet

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-66009 targets Beta Actin in WB, IP applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey samples.

Tested Applications WB
Cited Applications IP, WB
Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey
Cited Reactivityhuman, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2883836
Conjugate HRP
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.

实验方案

Product Specific Protocols
WB protocol for HRP Beta Actin antibody HRP-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Protein Cell

Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm

Authors - Kuisheng Liu
WB

Nat Commun

Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice

Authors - Nana Yan
humanWB

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
human,mouseWB

Nat Commun

SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.

Authors - Xiaowen Wang
humanWB

Bone Res

Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.

Authors - Dong Wang
mouseWB

JCI Insight

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling

Authors - Marta Bueno