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HRP-conjugated Beta Actin Monoclonal antibody 12 Publications

Mouse Monoclonal| Catalog number: HRP-66009 |CloneNo.: 2D4H5

Featured Product KD/KO validated

浓度: 1000 μg/ml

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Applications

Tested Applications:
WB
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Cited Applications:
WB


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WB analysis using HRP-66009

Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Species Specificity:
human, mouse, rat, hamster, zebrafish, monkey
Cited Species:
human, mouse, rat
Positive WB detected in:
Jurkat cells, HeLa, HepG2, HEK-293, HSC-T6, NIH/3T3, 4T1 cells
Recommended dilution:
WB : 1:2000-1:50000

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
actin, beta
Calculated molecular weight:
42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, HRP-66009, PS1TP5BP1, β actin

Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.

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