- Featured Product
- KD/KO Validated
HRP-conjugated Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for WB
Cat No : HRP-66009
|Positive WB detected in||Jurkat cells, HeLa, HepG2, HEK-293, HSC-T6, NIH/3T3, 4T1 cells|
|Western Blot (WB)||WB : 1:2000-1:50000|
|Sample-dependent, check data in validation data gallery|
The immunogen of HRP-66009 is Beta Actin Fusion Protein expressed in E. coli.
|Cited Applications||IP, WB|
|Tested Reactivity||human, mouse, rat, hamster, zebrafish, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Full Name||actin, beta|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||NM_001101|
|Uniprot||P60709||Gene ID (NCBI)||60|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.
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