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干货笔记
深度挖掘诱导多能干细胞（iPSCs）潜能：提升心肌细胞分化效能
作者：Jamuna Karanankattil Sukumaran（Proteintech公司高级科学家）
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How to Identify Activated Microglia
Proteintech experts share ways to quantify your microglial cell population
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7 Tips to successfully culture primary rodent neurons
Proteintech experts provide seven tips for improving the integrity of your primary neuronal cultures.
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How to choose the right secondary antibody
How to choose the best secondary antibody for your application.
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Overview of ChromoTek’s Nanobody-based tools for cancer research
ChromoTek’s research tools can help to understand cancer cell signaling pathways and cellular processes
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Breast Cancer Awareness Month
October is an annual health campaign to promote early detection, reduce risk factors and support patients
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Second Cancer After Breast Cancer
Explore why breast cancer survivors are more likely to develop a second cancer after the first one has been cured.
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What is the difference between pyroptosis and apoptosis?
Written by Mehak Passi, PhD Researcher at LMU Munich University
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Detecting low abundance proteins via Western Blot
Detecting low abundance proteins can be a challenge - here's how to optimise your Western Blot.
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How Nanobodies could advance Sars-CoV diagnostics
Teresa Wagner and Ulrich Rothbauer discuss the development of Nanobodies against SARS-CoV-2 and why Nanobodies are versatile alternatives to classical antibodies in SARS-CoV-2 research.
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The highly adaptive cytoskeleton
The cytoskeleton is a highly important structure present in the cytoplasm of all cells. Learn how this is integral for many cellular functions, and the many ways researchers can study the cytoskeleton in our blog.
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Mouse-on-mouse for Immunohistochemistry: tips and tricks
Performing IHC using a mouse derived antibody on mouse tissue can be daunting due to the potential for off-target staining. Learn how to mitigate these issues and perform mouse-on-mouse staining with confidence.
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Want to upgrade your immunofluorescence workflow? Go Direct!
Direct vs Indirect Immunofluorescence
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New ways to study the elusive exosome
Written by Poppy Nathan, Proteintech Field Marketing Representative and PhD Student at the University of Birmingham
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Try Proteintech Antibodies Germany
Free antibody samples from our bench to your bench
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Ask a Flow Cytometry Expert: Your Questions Answered
Dr. Gareth Howell, Senior Experimental Officer and Flow Core Facility Manager at the University of Manchester answers your questions.
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Navigating a PhD in a Pandemic
Guest blogger Francesca Lewns shares her tips and advice for navigating a PhD during a global pandemic
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To dye or not to dye? Understanding the different viability dyes available for flow cytometry
Dead cell exclusion in flow cytometry is crucial for reliable and accurate results. This blog will help you understand which reagents are best for your experiment, providing a comprehensive overview of the popular products available to help you find "another way to dye".
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Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments.
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Neuropilin-1 joins ACE2 as SARS-CoV-2 gateway
Landmark paper in Science detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry, using Proteintech and Chromotek antibodies.
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Intracellular Flow Cytometry Staining Protocol
Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.
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Interview with an early-career scientist: Dr Chaitali Mukherjee
Hear from an early-career scientist about her first-author publication in Cell Metabolism and her academic journey
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Exosomes: A big role for a small player
Exosomes uncovered. Recent research and the role of exosomes in disease
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What do you get when you buy a GMP-grade product?
GMP Manufacturing, benefits, and ISO certifications explained
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Comparing BCA, Bradford, and UV protein quantification methods for scientists
Understanding the pros and cons of BCA, Bradford, and UV methods
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Interview with an early-career scientist: Dr Nadav Weinstock
Hear from the lead author of a landmark paper in Neuron about his research into Krabbe Disease
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How to reduce autofluorescence
Understand the causes behind autofluorescence and how to deal with this problem.
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Serious about Serology: Understanding COVID19 Antibody Tests
Serious about serology: Understanding COVID19 Antibody Tests
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Cellular senescence and markers
How do cells come to be under "cellular arrest". Learn what is cell senescence and how it is relevant in disease.
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Neutralizing antibodies and their role in COVID-19 research
Weathering the Cytokine Storm
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How fellowships can advance your scientific career
Thoughts and advice to anyone thinking of applying for fellowships worldwide
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How to solve the genome DNA damage
DNA damage repair mechanisms: in the prevention of age-related diseases and tumorigenesis
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Dying to see what’s inside: Staining organelles
Visualizing the interior of organelles helps us to understand their molecular mechanisms and study cell abnormalities.
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Proteases for Western Blotting: Choosing the right tools for the job
Proteases are often labeled as “bad guys” that complicate our Western blot (WB) analyses. They cut up target proteins into unrecognizable pieces that do not resemble the expected results.
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Tips and tricks for successful MASS spec experiments
Troubleshooting guide to address the most common obstacles during MASS spec experiments.
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What is the difference between necrosis and apoptosis?
Cell death is a naturally occurring phenomenon in multicellular organisms. Cells die as a result of internal and external stimuli.
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Life Scientist Appreciation Week University of Chicago
June 17-21 2019. #CelebrateLifeScientists #LSAW
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Missing pieces: DNA damage
Various environmental and endogenous factors cause DNA damage, resulting in cell degeneration, aging, or tumorigenesis.
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IF imaging: Widefield versus confocal microscopy
How to answer the experimental question in the most efficient manner.
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MASS spec essentials
Mass spectrometry (mass spec; MS) is a powerful method for analyzing macromolecules.
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The state of the international scientist in the U.S.
Supporting foreign-born scientists contributing to biomedical research in the U.S.
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Why studying abroad makes sense: Scientists share their story
Perspectives on Emigrating for Academic Career Advancement
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CRISPR-Cas9, TALENs and ZFNs - the battle in gene editing
Technological advances are crucial for innovative biological research. ZFNs, TALENs, and CRISPR/CRISPR-associated Cas have revolutionized genome editing.
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QUANTITATIVE MIRNA PROFILING: HOW TO CHOOSE THE BEST RNA SEQUENCING METHOD?
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
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Are Mesenchymal Stem Cells (MSCs) true stem cells?
MSCs have great potential and clinical significance for tissue engineering. They are adult multipotent cells capable of differentiating to different types of tissues.
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7 secrets to a successful scientific presentation
Fight the fear of presenting your scientific research.
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FENS - the voice of European neuroscience
The 11th FENS Forum of Neuroscience took place in Berlin, Germany from July 7 to 11, 2018. Overall, it hosted over 7200 participants from more than 75 different countries.
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A closer look at Embryonic Stem Cells (ESCs) – biological concepts
Embryonic stem cells (ESCs) are derived from pre-implantation-stage embryos, usually from the inner cell mass of blastocyst-stage embryos.
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Life Scientist Appreciation Week
Life Scientist Appreciation Week. 21st - 25th June 2021 #LSAW21
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Why Human Cell Expressed Proteins Are Superior for Clinical Applications
For Humans...By Human Cells
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Cell fate commitment and the Waddington landscape model
Waddington’s epigenetic landscape has been used for decades to illustrate the progressive restriction of cell differentiation during development.
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Product Specific Protocols
Product specific protocols direct from our lab notebook to yours.
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How do I know if the antibody will cross-react?
Cross-reactivity occurs when an antibody raised against one specific antigen recognizes two antigens that have similar structural regions.
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5 Tips for immunostaining using multiple labels
A multiple immunostaining technique is a combination of individual antigen detections in order to study the expression and co-localization of two or more different antigens in the same sample.
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How does blood coagulate?
Blood is an essential fluid that carries out the critical functions of transporting oxygen and nutrients to our cells.
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ICF syndrome: A gene-silencing chromatin disorder
Immunodeficiency, Centromere region instability, Facial anomalies syndrome (ICF) is a rare autosomal recessive disease that damages the immune system.
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Induced pluripotent stem cells (iPSCs) and modeling neurodegenerative disease in the dish.
iPSCs and their potential for resetting the biological clock.
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Polyclonal vs. monoclonal antibodies
Both types have their unique advantages and disadvantages and can be used in a wide variety of applications.
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Epigenetic implications in brain functions - Neuroepigenetics
Environmental factors, stress, learning or drug exposure lead to active regulation of DNA structure in the nervous system.
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Immunotherapy: boosting the immune system to fight rare cancers
Immunotherapy, how does it work and what do we know so far?
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Rett syndrome - the second leading cause of mental retardation in women
In 1966, Rett syndrome was for the first time described as a clinical issue.
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Protein or peptide antigen? Advantages and disadvantages
Peptide versus Protein Immunogens
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Proteintech supports young scientist to attend EMBL conference
Proteintech supports young scientists – Non-Coding Genome EMBL conference Heidelberg, Germany, 13–16 Sep 2017
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Top Tips for a Successful Transition to a Postdoc Role
Advice and tips given by postdocs from all over the world.
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How to Write a Good Scientific Abstract
An abstract is a written statement describing key facts about your research project.
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How to be a great lab manager: 6 tips
Our advice to help you be a great lab manager and manage your time better.
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National Postdoc Appreciation Week
Celebrating the work of postdoctoral researchers together
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WNT Pathway - Defining the Canon
How did scientists figure out such a complicated pathway?
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The Complexity of Proteins
How do proteins have so much functional diversity based upon only 20 building blocks?
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Secondary Antibody Selection
How to choose the best secondary antibody for your application
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Alzheimer's Disease Signaling Pathway
Download or request a pathway poster for your lab
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MAPK Pathway: New Poster Available
Download or request a pathway poster for your lab
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Post Translational Modifications: An Overview
Nature’s escape from genetic imprisonment
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Caspase Cascade Pathway: New Poster Available
Download a pathway poster for your lab
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The Incubator Podcast Highlights
Season 2 is here! Here's a re-cap of the topics discussed in Season 1
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Cancer stem cells as a key to cure cancer
CSCs are cancerous yet have properties of stem cells: self-renewal and the ability to differentiate into multiple cell types that occurs in tumors.
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How To Optimize Your ELISA Experiments
Technical tips for better ELISA results
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GSK3 pathway poster
Request a printed copy of the GSK signaling pathway for your lab.
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Autophagy in the Pathogenesis of Disease
Aging, cancer, inflammation, infection, the microenvironment, and neurodegenerative disease
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9 Tips to optimize your IF experiments
Want to improve your IF experiments? Our 9 tech tips will help you achieve successful Immunofluorescence staining
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Special offer for newsletter sign up
Sign up to the Proteintech regular eNewsletter to receive a free lab survival kit.
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7 Tech Tips For Successful ChIP Experiments
New to ChIP or just want to improve? Our tech tips will help you achieve successful ChIP experiments
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Proteintech Product Reviews
Share your feedback with the research community and receive a $25 Amazon gift card or discount.
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Lysate Preparation: How Do I Optimize My Extraction?
How Do I Optimize My Extraction?
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Lysate Preparation: Why is RIPA Buffer Best for Western Blot
Why is RIPA Buffer Best for Western Blot?
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Antibody Comparison Sites: How to Find Your Perfect Antibody Faster
Find Your Perfect Antibody Faster
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Antibodies for Cilia Development
This article reviews a selection of proteins involved in cilia development, or ciliogenesis...
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A beginners guide to Immunohistochemistry
Are you starting Immunohistochemistry experiments for the first time?
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Loading Control Antibodies for Western Blotting
Technical tips and related products
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Immunofluorescence: Tips for Immunostaining Cultured Cells
Expert advice on immunostaining
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8 Top Tips for Subcellular Fractionation
8 technical tips for successful subcellular fractionation
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Mitochondria: More than just the powerhouse of the cell
Introduction to mitochondria
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Career Development Podcast: The Incubator
Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator.
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I Just Want Your Extra Time And Your...KISS-1
Anti-KISS-1 rabbit polyclonal antibody
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Seeing Clearly: Tips For Staining Cleared Tissue With Antibodies
Tips For Staining Cleared Tissue With Antibodies
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7 Tips For Optimizing Your Western Blotting Experiments
How to optimize your western blot protocol
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8 Top Tips For Immunoprecipitation
8 tips on how to perform better IP experiments.
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Mitochondria: Center of Cellular Signaling and Energetic Balance
Mitochondrial markers
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Recalling when and where: How the brain processes episodic memory
Introduction
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10 tips on how to best optimize siRNA transfection
siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and molecular pathways.
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Fast Phospho-rward: Making Phosphoprotein Antibodies
Phosphorylation is a key way that cells toggle activity of their equipment, playing integral roles in processes spanning from embryonic development to apoptosis.
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Molecular markers for liver cancer
The Proteintech blog explores molecular markers for Hepatocellular carcinoma
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Can man's best friend help cure cancer
Humans are not the only species on earth that develop cancer; our beloved pets can also succumb to the ‘Big C’ too.
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Can a Bacterial 'Brain' Control a Robot?
More and more, we are developing technology to precisely control biological systems....
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Making the connection between brain and body
We’re connected, from head to toe...
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Tech Tips | In search of low molecular weight proteins
Western Blot tips for low molecular weight proteins
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