Anti-IgG VHH Beads for IP
更完美的Protein A/G替代方案
Chromotek Anti-IgG VHH Beads for IP由偶联到beads上的纳米抗体组成。可实现精准的靶向捕获，获得更精准、纯净的结果。
优势：
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省心：无需考虑亲和力，告别Protein A/G选择困扰
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省时：仅需60min即可完成与IP捕获抗体的结合
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特异性高，背景干净：不与其他物种的IgG发生交叉反应，有效排除Protein A/G杂带干扰
可应用于：
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免疫沉淀(IP)
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免疫共沉淀(Co-IP)
抗IgG纳米抗体偶联beads
抗兔/鼠IgG通用，或靶向兔IgG、鼠IgG各亚型。 无论是明确所研究的亚型，还是处理未知的亚型抗体，这些产品均能完美替代Protein A/G，实现纯净、高效、稳定的免疫沉淀。
| 类型 | 产品类型 | 规格 | 特异性 | |
|---|---|---|---|---|
| anti-Rabbit/Mouse IgG | Agarose | Magnetic agarose | 1ml, 2ml, 5ml | Rabbit IgG, Mouse IgG1, 2a*, 2b*, 3* *kappa light chain |
| Rabbit | Agarose | Magnetic agarose | 1ml, 2ml, 5ml | Rabbit IgG |
| Mouse IgG1 | Agarose | Magnetic agarose | 1ml, 2ml, 5ml | Mouse IgG1 |
| Mouse IgG2a | Agarose | Magnetic agarose | 1ml, 2ml, 5ml | Mouse IgG2a |
| Mouse IgG2b | Agarose | Magnetic agarose | 1ml, 2ml, 5ml | Mouse IgG2b |
特异性靶向兔/鼠IgG，不与其他物种IgG反应
Cross-reactivity test and comparison to competitor Protein A and Protein G beads. Coomassie-stained SDS-PAGE gel showing input (IN) and bound (B) fractions for different beads incubated with 1:10 diluted sera of relevant model species. Unlike the tested protein A or G beads, bound fractions of Proteintech anti-rabbit IgG / anti-mouse IgG VHH agarose reveal no cross-reactivity to human, bovine, rat, guinea pig, or goat IgGs and only minor cross-reactivity to cyno monkey IgGs. For IN and B fractions 1% and 25% were loaded, respectively. Lanes of anti-IgG VHH beads are highlighted red. Results were reproducible for anti-rabbit IgG/ anti-mouse IgG Magnetic VHH Agarose.
杂带更少，结果更干净
IP of PCNA using 5 µg of anti-PCNA antibody (IgG1, 60097-1-Ig) by anti-rabbit IgG / anti-mouse IgG VHH beads. For Western blot analysis, the PCNA polyclonal antibody (10205-2-AP, 1:2000) was labeled using a conformation-specific HRP-conjugated secondary to avoid staining of heavy and light chain of the IP-antibody. In contrast to many competitors' protein A and G beads, clean pull-down of PCNA by Proteintech anti-rabbit IgG/ anti-mouse IgG VHH Beads (Ag. = Agarose; M.Ag. = Magnetic Agarose) facilitates unambiguous identification of the target protein. Other beads show leaching of protein A or protein G fragments into the final IP fractions, which can lead to binding of the detection antibody and unspecific signals, as reported previously (Grant et al., 2019, Biol Proced Online, doi: 10.1186/s12575-019-0095-z). For input (IN) and bound (B) fractions, 1% and 30% were loaded, respectively. Lanes of anti-IgG VHH beads are highlighted in red.
避免Protein A/G的干扰
使用纳米抗体偶联beads结合煮脱法，配合构象特异性二抗检测，既可以高效得到目的蛋白信号，又可以有效排除重/轻链和Protein A/G的杂带干扰.
Lane 1: IP of isotype control using Protein A beads (Boiling elution)；Lane 2: IP of 10530-1-AP using Protein A beads (Boiling elution)；Lane 3: Boiling elution of Protein A beads
Lane 4: IP of isotype control using Protein A beads (Acidic elution)；Lane 5: IP of 10530-1-AP using Protein A beads (Acidic elution)；Lane 6: Acidic elution of Protein A beads
Lane 7 and Lane 14: Input
Lane 8: IP of isotype control using anti-IgG VHH beads（mrIGa） (Boiling elution)；Lane 9: IP of 10530-1-AP using mrIGa (Boiling elution)；Lane 10: Boiling elution of mrIGa beads
Lane 11: IP of isotype control using mrIGa (Acidic elution)；Lane 12: IP of 10530-1-AP using mrIGa (Acidic elution)；Lane 13: Acidic elution of mrIGa
特异性靶向兔/鼠IgG
Binding efficiency and specificity of anti-rabbit IgG / anti-mouse IgG VHH agarose for rabbit and mouse IgG subclasses. Binding of antibodies of different subtypes was tested at increasing concentrations from HEK293T cell lysate. The high capacity of the anti-IgG VHH beads allows for efficient binding of all IgGs tested.
Binding efficiency and specificity of anti-rabbit IgG / anti-mouse IgG magnetic VHH agarose for rabbit and mouse IgG subclasses. Binding of antibodies of different subtypes was tested at increasing concentrations from HEK293T cell lysate. The high capacity of the anti-IgG VHH beads allows for efficient binding of all IgGs tested.
成功对大分子靶蛋白复合物进行免疫共沉淀
Co-IP of MCM complex via pulldown of MCM6 using 5 µg of anti-MCM6 antibodies and anti-rabbit IgG / anti-mouse IgG VHH agarose. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated using both a mouse IgG1 (67989-1-Ig) and a rabbit (13347-2-AP) MCM6 antibody, as shown by western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded. For bound (B) fraction and bound fraction of isotype control antibody (BISO), 20% was loaded. Product codes for PTG-antibodies are provided in parentheses. BISO is an isotype control used as negative control (ms1: 66260-1-Ig; rb: 30000-0-AP). Results were reproducible for anti-rabbit IgG/ anti-mouse IgG Magnetic VHH Agarose.
