基于纳米抗体的试剂
纳米抗体是已知的最小抗体（12-15 kDa ）。它们是来自羊驼、美洲驼或骆驼的抗原结合域或重链抗体的可变结构域（VHHs）.
ChromoTek纳米抗体的试剂经过验证，重组表达，并可直接使用。由于它们分子小和单链的特性，它们在免疫沉淀、免疫荧光、活细胞成像、生物传感器检测和蛋白质纯化等应用中提供了比传统IgG抗体更高水平的性能.
Chromotek is now part of Proteintech
All ChromoTek Nanobody-based reagents are now available on ptglab.com.
Chromobodies
Chromobodies® 是一种荧光纳米探针，通过将羊驼单域抗体VHH 与荧光蛋白的基因序列融合在一个重组质粒来实现内源性靶标蛋白在活细胞内的可视化和实时分析.
Nano-Booster &
Nano-Label
Nano-Booster &Nano-Label是一类非常小的荧光探针，用于共聚焦和超分辨率显微镜。它们由与荧光染料偶联的纳米体组成.
Nano-CaptureLigands
Nano-CaptureLigands 是生物素偶联的纳米抗体 /VHH。经过优化，可特异性固定人、小鼠和兔偶联链霉亲和素/亲和素的抗体.
Spot-Tag System
ChromoTek专有的肽标签系统，用于免疫沉淀、免疫荧光和蛋白质纯化。根据应用的不同，Spot Nanobodies与琼脂糖、珠子或荧光染料结合.
Immunoprecipitation
Nanobody based reagents show less background, i.e., no heavy and light antibody chains in SDS/PAGE and Western blot.
Immunofluorescence
Due to the small size of Nanobodies, higher image resolution can be achieved.
Live Cell Imaging
Chromobodies® visualize endogenous cellular structures and processes in live cells and real-time.
Western Blotting
Fluorescently labeled Nanobodies and antibodies allow for multiplexing.
Super-resolution Microscopy
The small size of Nanobodies enables better tissue penetration and decreases the distance between epitope and label.
Mass Spectrometry
Nanobody based tools enable for high reproducibility and low background.
Protein Purification
Affinity resins with Nanobodies allow for effective one-step protein purification.
Spot Capture and Detection System
Universal capture and detection system based on Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.
On-bead Enzyme Assays
The enzymatic activity can be assessed directly after immunoprecipitation from a cell lysate with Nanobodies.
High Content Analysis
Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time.
Ligand Capture and Affinity Determination
Directed immobilization of antibodies for characterization of biomolecular interactions such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI).
ELISA
An Enzyme-linked immunosorbent assay (ELISA) is method for the detection of a target molecule in a liquid sample.
Products
GFP-Trap® Agarose
An affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
| Cat No: | Publications |
| gta | 2,863 |
V5-Trap® Magnetic Agarose
An affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/ VHH coupled to magnetic agarose beads
| Cat No: | Applications |
| v5tma | IP, CoIP, ChIP, RIP |
Myc-Trap® Agarose
An affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
| Cat No: | Publications |
| yta | 77 |
Alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647
Consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
| Cat No: | Applications |
| sms1AF647-1 | IF, WB, FC |
Spot-Label ATTO594
Anti-Spot-Tag VHH/ Nanobody conjugated to ATTO594.
| Cat No: | Applications |
| eba594 | IF, WB |
Actin-Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
| Cat No: | Publications |
| acr | 7 |
RFP-Trap® Magnetic Particles M-270
RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
| Cat No: | Applications |
| rtd | IP, CoIP |
Articles
About Nanobodies
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.
Non visible textSpot Capture & Detection System
Universal capture and detection system based on Spot-Tag®, an inert 12 amino acid peptide-tag and Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.
Non visible textFree Samples
Request a free sample and test the GFP-Trap & other Nano-Traps yourself.
Non visible textAbout Nanobodies
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.