基于纳米抗体的试剂

纳米抗体是已知的最小抗体(12-15 kDa )。它们是来自羊驼、美洲驼或骆驼的抗原结合域或重链抗体的可变结构域(VHHs).

ChromoTek纳米抗体的试剂经过验证,重组表达,并可直接使用。由于它们分子小和单链的特性,它们在免疫沉淀、免疫荧光、活细胞成像、生物传感器检测和蛋白质纯化等应用中提供了比传统IgG抗体更高水平的性能.

Chromotek is now part of Proteintech

All ChromoTek Nanobody-based reagents are now available on ptglab.com and chromotek.com.

Nano-Traps

Nano-Traps是快速、可靠、高效的一步免疫沉淀和免疫共沉淀的理想选择。Nano-Traps由与琼脂糖珠或磁珠偶联的纳米体组成.

Nano-Secondaries

Nano Secondaries® 是一种可以获得更高分辨率,更清晰图像的新型二抗。该二抗是荧光染料欧联的纳米抗体.

Chromobodies

Chromobodies® 是一种荧光纳米探针,通过将羊驼单域抗体VHH 与荧光蛋白的基因序列融合在一个重组质粒来实现内源性靶标蛋白在活细胞内的可视化和实时分析.

Nano-Booster &
Nano-Label

Nano-Booster &Nano-Label是一类非常小的荧光探针,用于共聚焦和超分辨率显微镜。它们由与荧光染料偶联的纳米体组成.

Nano-Caps

Nano-Caps®是纳米抗体 /VHH 偶联的琼脂糖珠,可以高效的进行一步法纯化蛋白.

Nano-CaptureLigands

Nano-CaptureLigands 是生物素偶联的纳米抗体 /VHH。经过优化,可特异性固定人、小鼠和兔偶联链霉亲和素/亲和素的抗体.

Nanobodies/VHHs

适用于通过NHS酯反应与染料、生物素、珠子、表面等偶联.

Spot-Tag System

ChromoTek专有的肽标签系统,用于免疫沉淀、免疫荧光和蛋白质纯化。根据应用的不同,Spot Nanobodies与琼脂糖、珠子或荧光染料结合.

Immunoprecipitation

Nanobody based reagents show less background, i.e., no heavy and light antibody chains in SDS/PAGE and Western blot.

Immunofluorescence

Due to the small size of Nanobodies, higher image resolution can be achieved.

Live Cell Imaging

Chromobodies® visualize endogenous cellular structures and processes in live cells and real-time.

Western Blotting

Fluorescently labeled Nanobodies and antibodies allow for multiplexing.

Super-resolution Microscopy

The small size of Nanobodies enables better tissue penetration and decreases the distance between epitope and label.

Mass Spectrometry

Nanobody based tools enable for high reproducibility and low background.

Protein Purification

Affinity resins with Nanobodies allow for effective one-step protein purification.

Spot Capture and Detection System

Universal capture and detection system based on Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.

On-bead Enzyme Assays

The enzymatic activity can be assessed directly after immunoprecipitation from a cell lysate with Nanobodies.

High Content Analysis

Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time.

Ligand Capture and Affinity Determination

Directed immobilization of antibodies for characterization of biomolecular interactions such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI).

ELISA

An Enzyme-linked immunosorbent assay (ELISA) is method for the detection of a target molecule in a liquid sample.