ChromoTek GFP-Trap® Agarose

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
Cat No. gta
引用文献(4188)

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

规格: 

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Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

DescriptionImmunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity25-30 µg of recombinant GFP per 25 µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_2631357
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Size25ul/reactions (eg:20rxns=500ul slurry)

Publications

ApplicationTitle
IP

Nature

Mechanisms of RALF peptide perception by a heterotypic receptor complex

Authors - Yu Xiao

Nature

Principles of mitoribosomal small subunit assembly in eukaryotes

Authors - Nathan J Harper
IP

Nature

Decoding chromatin states by proteomic profiling of nucleosome readers

Authors - Saulius Lukauskas
IP

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
IP

Cell

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Authors - Lina Herhaus
IP

Cell

The phosphorylation landscape of infection-related development by the rice blast fungus

Authors - Neftaly Cruz-Mireles