Validation Data Gallery
Product Information
GFP-Trap® Multiwell Plate for immunoprecipitation (IP) of GFP-tagged proteins.
| Description | Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody immobilized in 96 well plates.
• Up to 96 samples in parallel • No centrifugation needed • Pre-blocked • Convenient handling • Suitable also for IP of very large proteins/complexes • Low background • High binding efficiency • Extraordinarily robust • Reproducible results • Validated |
| Applications | IP, CoIP, ELISA |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 200-500 ng of recombinant GFP per well |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 10 mM DTT, 10 mM β-mercaptoethanol, 10 mM TCEP, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.2 % SDS, 2 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Do not freeze! |
Documentation
| Datasheet |
|---|
| Protocol GFP-Trap Multiwell Plates (PDF) |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) |
| GFP-Trap Specificity |
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| Fluorescent protein specificity table |
Publications
| Application | Title |
|---|---|
Nature Distinct proteostasis circuits cooperate in nuclear and cytoplasmic protein quality control. | |
Immunity The Chaperone UNC93B1 Regulates Toll-like Receptor Stability Independently of Endosomal TLR Transport. | |
New Phytol VAMP721a and VAMP721d are important for pectin dynamics and release of bacteria in soybean nodules. | |
Hum Mol Genet DNMT1 mutations found in HSANIE patients affect interaction with UHRF1 and neuronal differentiation. | |
Mol Cell Proteomics A Double-Barrel Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) System to Quantify 96 Interactomes per Day. |