ChromoTek GFP-Trap® Multiwell Plate
GFP-Trap® Multiwell Plate for immunoprecipitation (IP) of GFP-tagged proteins.
Cat No : gtp
Synonyms
Validation Data Gallery
Product Information
GFP-Trap® Multiwell Plate for immunoprecipitation (IP) of GFP-tagged proteins.
| Applications | IP, CoIP, ELISA |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 1 pM |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
| Coupled VHH | Anti-GFP single domain antibody (VHH, Nanobody) |
| Conjugate | Unconjugated |
| Beads | Immobilized in 96 multiwell plate (polystyrene, transparent), pre-blocked with BSA and Casein hydrolysate, lyophilized |
| Binding capacity | 200-500 ng of recombinant GFP per well |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| Protocol |
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| Protocol GFP-Trap Multiwell Plates (PDF) |
| White papers |
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| GFP-Trap® as affinity tag |
| How to plan an Immunoprecipitation using the GFP-Trap |
| Split Fluorescent Protein Technology (PDF) |
| Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) |
| Brochure |
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| GFP-Trap product brochure |
| GFP-Trap Specificity |
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| Fluorescent protein specificity table |
Publications
| Application | Title |
|---|---|
PLoS One The biochemical properties of the Arabidopsis ecto-nucleoside triphosphate diphosphohydrolase AtAPY1 contradict a direct role in purinergic signaling. | |
BMC Plant Biol The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space. | |
Sci Rep Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments. | |
Front Mol Biosci A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1. | |
J Biol Chem Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein. |