▲Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
▲Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
▲1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®488 Anti-Human Phospho-STK11 (Thr189) (CL488-80127, Clone:5J9), or 0.13 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.