FlexAble logo 抗体标记试剂盒

Flexable 抗体标记试剂盒

Any antibody. Any color. Any time.

FlexAble logo

FlexAble系列是Proteintech研发的一种新型抗体标记试剂盒,采用独有的FlexLinker®抗体标记技术专利,能在任何抗体缓冲液条件下,快速将荧光染料、酶、以及其他分子与抗体结合。

  • 与任何品牌一抗都兼容
  • 单次最少可标记0.5ug抗体
  • 适用于任何抗体浓度和配方
  • 标记过程中抗体无损失
  • 两步法10分钟快速标记
  • 无需额外设备或更换缓冲液
FlexAble logo

FlexAble系列是Proteintech研发的一种新型抗体标记试剂盒,采用独有的FlexLinker®抗体标记技术专利,能在任何抗体缓冲液条件下,快速将荧光染料、酶、以及其他分子与抗体结合。

  • 与任何品牌一抗都兼容
  • 适用于任何抗体浓度和配方
  • 两步法10分钟快速标记
  • 单次最少可标记0.5ug抗体
  • 标记过程中抗体无损失
  • 无需额外设备或更换缓冲液

FlexAble抗体标记试剂盒是通过高亲和力的FlexLinker®以非共价结合的形式标记抗体。FlexLinker®能在几分钟之内与抗体紧密结合,并且一旦结合就不会轻易从抗体上释放。标记体系中过量的、未结合抗体的FlexLinker®可通过加入猝灭剂FlexQuencher将其中和掉。

经充分测试,FlexAble标记抗体对抗体与靶标的结合没有影响,同时也不会造成抗体损失。

Antibody labeling with Proteintech's FlexAble kit is achieved by using a high affinity non-covalent linker that binds tightly to the antibody within minutes and remains attached indefinitely.

FlexAble抗体标记试剂盒可以与任何供应商的一抗兼容,不论抗体供应商品牌、抗体浓度、以及抗体缓冲液体系。高浓度抗体标记无需稀释,低至0.06mg/mL浓度的抗体也能被有效标记。

FlexAble抗体标记试剂盒适用于任何抗体制剂体系,无需在标记前纯化或者更换缓冲液体系,不会造成抗体的损失,也不受抗体浓度、稳定剂(甘油、BSA等)、含胺缓冲液(Tris等)、防腐剂(叠氮化钠等)等成分的干扰。

Anti-CD3 antibody, previously stored in the indicated concentration of glycerol, was efficiently labeled with FlexAble Antibody Labeling Kit regardless of the glycerol concentration during labeling. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 650 Kit (KFA023) . Cells were gated for live singlet lymphocytes.

FlexAble标记抗体仅需一把移液器,两个步骤,10分钟,即可轻松完成抗体标记。

第一步,将一抗、FlexLinker和FlexBuffer混合在一起,孵育5分钟;第二步,加入FlexQuencher以中和未结合的FlexLinker,并再次孵育5分钟。标记完成,管中的即为标记好的抗体,可以直接用于下一步的实验。

Step-by-step workflow of Proteintech's FlexAble antibody labeling kit shows 30 seconds hands-on time and ready-to-use in less than 10 minutes

FlexAble抗体标记试剂盒可以简化免疫荧光(IF)、蛋白印迹(WB)和流式细胞术(FC)中的多重染色流程,通过在实验前10分钟内完成所有抗体的标记工作,然后同时用所有标记的抗体进行多重染色,简化实验流程,节省孵育时间。

1.使用不同种类的一抗,可以同时在一个管子中用不同的FlexAble抗体标记试剂盒对不同的一抗进行标记。

Primary antibodies of different species can be labeled simultaneously in the same tube with FlexAble antibody labeling kits. Immunofluorescence of rat brain tissue stained using NueN antibody, TUBB3 antibody and GFAP antibody

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN antibody (66836-1-Ig) labeled with FlexAble CoraLite Plus 650 Kit (KFA023, magenta), anti-TUBB3 antibody 66375-1-Ig) labeled with FlexAble CoraLite Plus 550 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).

2.使用相同种类的一抗,可以在不同管子中,使用不同的FlexAble抗体标记试剂盒对不同靶标一抗进行标记,然后将标记好的多个靶标一抗进行混合使用。

FlexAble provides the easiest way to multiplex antibodies from the same species and isotype immunofluorescence staining of HeLa cells with TDP43 antibody,] and Lamin B1 antibody

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 antibody (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 antibody (11802-1-AP) labeled with FlexAble CoraLite Plus 550 Kit (KFA002, red), and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite Plus 650 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

3.FlexAble抗体标记试剂盒标记的抗体,不仅能单独使用,也能配合其他商业化的直标抗体或者其他商业化的一抗二抗(间标)联合使用。

Flexable 抗体标记试剂盒列表

偶联荧光染料 规格 适配抗体类型
CoraLite 488 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 550 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 650 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 750 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
Conjugate Size
CoraLite 488 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 550 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 650 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1
CoraLite Plus 750 10 rxn, 50 rxn, 200 rxn Rabbit IgG, Mouse IgG1