FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG

Application

IF, FC, WB

Reactivity

Rabbit

Conjugate

CoraLite® Plus 488

Cat No : KFA001

Print Protocol



产品介绍

FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG is a novel antibody labeling kit that uses an affinity linker to conjugate CoraLite® Plus 488 in any buffer condition to rabbit IgG primary antibodies from any supplier. One labeling reaction requires only 0.5µg of antibody – regardless of the antibody concentration. Label your rabbit IgG antibody in <10 min without any additional equipment.


Product name                

FlexAble CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG                

Assay type                

Antibody labeling                

Tested applications                

IF, FC, WB                

Species Reactivity                

Rabbit IgG                

Antibody amount per labeling reaction            

0.5 µg antibody               

Conjugate                

CoraLite® Plus 488               

Excitation / Emission maxima wavelengths               

500 nm / 520 nm                


Component            10 rxns50 rxns
           
4×50 rxns            
CoraLite® Plus 488 FlexLinker for Rabbit IgG10 μL50 μL4×50 μL
FlexQuencher for Rabbit IgG20 μL100 μL4×100 μL
FlexBuffer100 μL500 μL4×500 μL
Manual1 Copy
Protocol Magnet1 Piece


Storage Condition

Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.

FAQ

Q: What are the FlexLinker, FlexQuencher and FlexBuffer?

A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.


Q: What is the largest quantity I can label?

A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.


Q: What is the lowest concentration of my primary antibody that I can use?

A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.


Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?

A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.


Q: How many different primary antibodies can I label with one kit?

A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.


Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?

A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies. 


More FAQs


Publications

ApplicationTitle
WB

Stem Cell Res Ther

A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models

Authors - Jeremy D Burgess
WB

Res Sq

A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models

Authors - Jeremy D Burgess

Elife

Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis

Authors - Sotaro Ichinose
IF

Front Cell Neurosci

K+/Cl- cotransporter 2 (KCC2) and Na+/HCO3- cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylation

Authors - Abhishek Pethe

Mol Cell Proteomics

Interactome analysis identifies the role of BZW2 in promoting endoplasmic reticulum-mitochondrial contact and mitochondrial metabolism

Authors - George Maio
FC

Adv Sci (Weinh)

BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity

Authors - Zhiyong Cai