Spot捕获及检测系统
用于免疫沉淀、蛋白质纯化和免疫荧光
ChromoTek专有的、新型Spot-System是第一个用于捕获和检测应用的肽标签特异性纳米体。它由Spot-Tag®（一种惰性的12氨基酸肽标签（PDRVRAVSHWSS））和Spot-Nanobody组成，特异性地与具有高亲和力的Spot标记蛋白质结合。
应用和工具:
- 免疫沉淀（IP）/ Co-IP：Spot-Trap®
- Co-IP/MS /MS: Spot-Trap and iST Spot-Trap Kit
- 蛋白质纯化: Spot-Trap®
- 免疫荧光: Spot-Label®
- 蛋白质印迹: Spot-tag antibody [28a5]
| Product | Formats (Product citations in parentheses) |
|---|---|
| Spot-Cap | Agarose beads |
| Spot-Label® | ATTO488, ATTO594, Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 |
| Spot-Trap® | Agarose (3), Magnetic Agarose (6), Magnetic Particles M-270 |
| Spot VHH | Unconjugated |
| Spot-tag® antibody (28A5) | Unconjugated |
| Spot-Tag Vectors for cloning | ev-1, ev-2, ev-3, ev-4, ev-5, ev-6, ev-7, ev-8 |
| Spot Vectors - positive controls | ev-31, ev-32, ev-33 |