ChromoTek Spot VHH, recombinant binding protein
Alpaca anti-Spot VHH, purified recombinant binding protein
Cat No : etb
Synonyms
Validation Data Gallery
Product Information
Alpaca anti-Spot VHH, purified recombinant binding protein
| Description | Alpaca anti-Spot VHH, purified recombinant binding protein binds extraordinary stable & reliable to Spot-Tag®:
• Stable up to 65 °C, 10 mM DTT, 0.75 M Guanidinium•HCl, 2 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.1 % SDS, 1 % Triton X-100, 1 % Dexoycholate • pH 4-10 • Fulfills highest requirements for antibody validation • Structure and function are characterized |
| Applications | Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction Spot VHH contains a sortase-tag and a His-tag at its C terminus: LPETGHHHHHH. This sequence includes the sortase recognition motif LPETG, for conjugation. In addition, bivalent Spot-VHH may be detected by an anti-His antibody. The Spot VHH corresponds to the “BC2-Nanobody” used by Virant et al in the publication "Nature Communications" (2018) doi:10.1038/s41467-018-03191-2 refdoi:10.1038/s41467-018-03191-2 |
| Specificity/Target | Spot-Tag® sequence PDRVRAVSHWSS |
| Conjugate | Unconjugated |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 6 nM |
| Molecular Weight | 30.3 kDa |
| RRID | AB_2827572 |
| Storage Buffer | 25 mM TAPS pH8,5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze. |
| Spot-Tag® Origin | Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin |
| Cross Reactivity | The Spot-Nanobody shows minimal cross reactivity with beta-catenin. |
Publications
| Application | Title |
|---|---|
Sci Adv Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging. | |
iScience ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodes. | |
Cells Cep192, a Novel Missing Link between the Centrosomal Core and Corona in Dictyostelium Amoebae. | |
Cells The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection. | |
Life Sci Alliance The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair. |