ChromoTek pSpot2 vector
The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.
Cat No : ev-2
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.
| Applications | Protein expression |
| Vector type | bacterial expression vector |
| Promoter | T7 |
| Replication | pBR322 |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) C-terminal |
| Selection | kanamycin |
Publications
| Application | Title |
|---|---|
Cells The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection. | |
Int J Mol Sci Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice |