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Validation Data Gallery

  • SPOT tag related products

    SPOT tag related products

  • Left: HeLa cells transiently expressing Spot-Tag?-Lamin B1 were immunostained with Spot-Label? Alexa Fluor? 488 (ebAF488, 1:800). Right: Control staining of untransfected cells. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective

    Left: HeLa cells transiently expressing Spot-Tag?-Lamin B1 were immunostained with Spot-Label? Alexa Fluor? 488 (ebAF488, 1:800). Right: Control staining of untransfected cells. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective

ChromoTek Spot-Label® Alexa Fluor® 488

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.
Cat No. ebAF488
引用文献(1)

Specificity

Spot-Tag®

Applications

IF

Conjugate

Alexa Fluor® 488

Host

Alpaca

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag

Formats:  Alexa Fluor® 488
规格价格库存

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Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

DescriptionImmunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

• Capture and detection tag without compromises in applications

• Higher image resolution

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Strong avidity effect from bivalent form of Spot-VHH

• Fulfills highest requirements on antibody validation: structure and function characterized

ApplicationsIF
HostAlpaca
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS)
ConjugateAlexa Fluor® 488
Physical StateLiquid
Suggested DilutionIF: 1:800
Type Nanobody
ClassRecombinant
ClonalityMonoclonal
CloneCTK0216
Calculated MW30.3 kDa
RRIDAB_2889374
ImmunogenBeta-catenin, engineered epitope
FormatRecombinant alpaca single domain antibody, bivalent
Affinity (KD)Dissociation constant KD of 6 nM
DOL1 fluorophore per bivalent Nanobody, site-directed conjugation
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.
Excitation/ EmissionExcitation max: 490 nm, Emission max: 525 nm
Storage BufferPBS, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
Size10 μL; 50 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

EMBO Rep

The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans

Authors - Prabhu Sankaralingam
View Article