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ChromoTek Spot-Label® ATTO488

anti-Spot-Tag® VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag®

Applications

IF

Conjugate

ATTO 488

Host

Alpaca

Cat No : eba488

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label

Validation Data Gallery

  • SPOT tag related products

    SPOT tag related products

Product Information

anti-Spot-Tag® VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

DescriptionImmunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

• Capture and detection tag without compromises in applications

• Higher image resolution

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Strong avidity effect from bivalent form of Spot-VHH

• Fulfills highest requirements on antibody validation: structure and function characterized

ApplicationsIF
HostAlpaca
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS)
ConjugateATTO 488
Physical StateLiquid
Suggested DilutionIF: 1:1,000
Type Nanobody
ClassRecombinant
RRIDAB_2827569
Affinity (KD) Dissociation constant KD of 6 nM
Storage Buffer1x PBS, 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Publications

ApplicationTitle
IF

Physiol Plant

Protein Detection and Localization in Plant Cells Using Spot-Tagging

Authors - Andriani Mentzelopoulou
View Article