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ChromoTek Spot-Trap® Agarose

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

Specificity

Spot-Tag®

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Cat No : eta

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

Validation Data Gallery

  • Immunoprecipitation of Spot-tagged protein.

    Immunoprecipitation of Spot-tagged protein.

  • Immunoprecipitation of Spot-tagged protein. Input (I), non-bound (FT) and bound (B), Coomassie staining and Western blotting. Western blot indicates high effectivity.

    Immunoprecipitation of Spot-tagged protein. Input (I), non-bound (FT) and bound (B), Coomassie staining and Western blotting. Western blot indicates high effectivity.

  • Elution of Spot-tag fusion protein. Input (I), non-bound (FT) and eluted (E1, E2 and E3) fractions. Elutions with Spot-peptide.

    Elution of Spot-tag fusion protein. Input (I), non-bound (FT) and eluted (E1, E2 and E3) fractions. Elutions with Spot-peptide.

  • Application of Spot-tag related products.

    Application of Spot-tag related products.

Product Information

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

DescriptionImmunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to agarose beads.

• No contaminating heavy and light antibody chains

• Ready-to-use

• Recombinantly expressed without batch-to-batch variation

• Well-characterized and validated

• High stability allows for stringent washing

• Extraordinary low background compared to other affinity resins

• Short incubation time of 30-60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSpot-Tag sequence PDRVRAVSHWSS
Binding capacity17.5 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.1 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Compatibility with mass spectrometryThe Spot-Trap® is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_2827590
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Publications

ApplicationTitle
IP

J Biol Chem

Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation.

Authors - Valerie L Su
View Article
IP

Biomolecules

Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.

Authors - Teresa R Wagner
View Article

J Cell Sci

30 years of nanobodies - an ongoing success story of small binders in biological research

Authors - Desiree I Frecot
View Article

J Cell Sci

Identification of distinct active pools of yeast serine palmitoyltransferase in sub-compartments of the ER

Authors - Bianca M Esch
View Article

Nature

TDP-43 forms amyloid filaments with a distinct fold in type A FTLD-TDP

Authors - Diana Arseni
View Article
IP

EMBO J

Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease

Authors - Katarzyna M Grochowska
View Article