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ChromoTek Spot-Trap® Magnetic Particles M-270 Kit

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

Spot-Tag®

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat No : etdk

Synonyms

etdk, etdk-20, Spot Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270 Kit, Spot-Trap® Magnetic Particles M-270 Kit, etdk-20, PDRVRAVSHWSS, spot, spot tag, spot-tag, Spot-Trap® Magnetic Particles M-270

Validation Data Gallery

  • Spot-Trap Magnetic Particles M-270 pull-down.

    Spot-Trap Magnetic Particles M-270 pull-down.

  • Immunoprecipitation (IP) of GFP with Spot-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

    Immunoprecipitation (IP) of GFP with Spot-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

  • Application of Spot-tag related products.

    Application of Spot-tag related products.

  • Cartoon to visualize the binding of large protein with Spot-tag (Spot-tag (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (tag (green) + protein of interest (POI, blue) to Spot-Nanobody (dark green) of Spot-Trap Magnetic Agarose or Spot-Trap Magnetic Particles M-270.

    Cartoon to visualize the binding of large protein with Spot-tag (Spot-tag (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (tag (green) + protein of interest (POI, blue) to Spot-Nanobody (dark green) of Spot-Trap Magnetic Agarose or Spot-Trap Magnetic Particles M-270.

Product Information

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionThe Spot-Trap Magnetic Particles M-270 Kit contains Spot-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Spot-tagged proteins and their interacting factors.

• No contaminating heavy and light antibody chains

• Extraordinary low background compared to other affinity resins

• Recombinantly expressed without batch-to-batch variation

• Well-characterized and validated

• Compatible with harsh buffer conditions

• Suitable for downstream mass spec analysis

• Short incubation time of 30-60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSpot-Tag ® sequence PDRVRAVSHWSS
Binding capacity2.25 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Compatibility with mass spectrometryThe Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2861252
Storage BufferPBS with 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Kit components

ComponentDescription
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Spot-Trap Magnetic Particles M-27020 reactions (500 µL)