ChromoTek pSpot2 GFPSpot-Tag vector
The plasmid pSpot2_GFP is a bacterial expression vector encoding Aequorea victoria Enhanced Green fluorescent protein (EGFP) fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of EGFP. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
Vector type
Bacterial expression vector
Promoter
T7
Reporter
Spot-Tag® C-terminal
Cat No : ev-32
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot2_GFP is a bacterial expression vector encoding Aequorea victoria Enhanced Green fluorescent protein (EGFP) fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of EGFP. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
| Description | The plasmid pSpot2_GFP is a bacterial expression vector encoding Aequorea victoria Enhanced Green fluorescent protein (EGFP) fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of EGFP. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system. |
| Applications | Protein expression |
| Vector type | Bacterial expression vector |
| Promoter | T7 |
| Replication | pMB1 |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) C-terminal |
| Selection | Kanamycin |