ChromoTek pSpot-Tag-Actin vector
The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.
Vector type
Mammalian expression vector
Promoter
CMV
Reporter
Spot-Tag® N-terminal
Cat No : ev-31
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.
| Description | The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human beta-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system. |
| Applications | Protein expression1 |
| Vector type | Mammalian expression vector |
| Promoter | CMV |
| Replication | pMB1 |
| Reporter | Spot-Tag® (PDRVRAVSHWSS)N-terminal |
| Codon usage | Mammalian |
| Selection | Kan/Neo |