- Featured Product
- KD/KO Validated
CCDC111/PRIMPOL Polyclonal antibody
CCDC111/PRIMPOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 8 publications below |
产品信息
29824-1-AP targets CCDC111/PRIMPOL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | CCDC111/PRIMPOL fusion protein Ag31478 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | coiled-coil domain containing 111 |
Synonyms | CCDC111, FLJ33167, hPrimpol1, PRIMPOL |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC064600 |
Gene Symbol | CCDC111/PRIMPOL |
Gene ID (NCBI) | 201973 |
RRID | AB_2918349 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96LW4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
IHC protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
IF protocol for CCDC111/PRIMPOL antibody 29824-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress | ||
Nat Commun Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases | ||
Int J Mol Sci CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin | ||
bioRxiv CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps | ||
Nucleic Acids Res USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress | ||
Cell Rep FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition
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