• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC and More (1)

Conjugate

Unconjugated

CloneNo.

HI30

Cat no : 65109-1-Ig

Print datasheet

Synonyms

L CA, HI30, GP180, EC:3.1.3.48, CD45R

验证数据展示

  • FC experiment of human PBMCs using 65109-1-Ig

    FC experiment of human PBMCs using 65109-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45 (65109-1-Ig, Clone:HI30) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

  • IF Staining of Jurkat using 65109-1-Ig

    IF Staining of Jurkat using 65109-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC Application Description
Cited ApplicationsFC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Human peripheral blood leucocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms L CA, HI30, GP180, EC:3.1.3.48, CD45R
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2918407
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08575
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 65109-1-IgDownload protocol
FC protocol for CD45 antibody 65109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
View Article
humanIF

Cell Discov

Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics

Authors - Min Tang
View Article