验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 1 publications below |
产品信息
65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC Application Description |
| Cited Applications | FC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Human peripheral blood leucocytes 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Synonyms | L CA, HI30, GP180, EC:3.1.3.48, CD45R |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2918407 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CD45 antibody 65109-1-Ig | Download protocol |
| FC protocol for CD45 antibody 65109-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
Cell Discov Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics |