验证数据展示
经过测试的应用
Positive IF/ICC detected in | Jurkat cells |
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
FC | See 1 publications below |
产品信息
65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC Application Description |
文献引用应用 | FC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Human peripheral blood leucocytes 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | protein tyrosine phosphatase, receptor type, C |
别名 | L CA, HI30, GP180, EC:3.1.3.48, CD45R |
GenBank蛋白编号 | BC014239 |
基因名称 | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
RRID | AB_2918407 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P08575 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
Product Specific Protocols | |
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IF protocol for CD45 antibody 65109-1-Ig | Download protocol |
FC protocol for CD45 antibody 65109-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
Cell Discov Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics |