验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CPY5-65109 targets CD45 in FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
经测试反应性 | human |
免疫原 | Human peripheral blood leucocytes 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | protein tyrosine phosphatase, receptor type, C |
别名 | LCA, L CA, HI30, GP180, EC:3.1.3.48 |
GenBank蛋白编号 | BC014239 |
基因名称 | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
RRID | AB_3673820 |
偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
最大激发/发射波长 | 482 nm / 690 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P08575 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
Product Specific Protocols | |
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FC protocol for PerCP-Cyanine5.5 CD45 antibody CPY5-65109 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |