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验证数据展示

  • FC experiment of human PBMCs using CL647-65109

    FC experiment of human PBMCs using CL647-65109

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone: HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • IF Staining of Jurkat using CL647-65109

    IF Staining of Jurkat using CL647-65109

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD45 antibody (CL647-65109, Clone: HI30 ) at dilution of 1:200.

CoraLite® Plus 647 Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for IF/ICC, FC
Cat No. CL647-65109

产品说明书

CloneNo. HI30

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IF/ICC, FC

别名

L CA, HI30, GP180, EC:3.1.3.48, CD45R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65109-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65109 targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Human peripheral blood leucocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 L CA, HI30, GP180, EC:3.1.3.48, CD45R
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2883687
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP08575
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD45 antibody CL647-65109Download protocol
FC protocol for CL Plus 647 CD45 antibody CL647-65109Download protocol
Standard Protocols
Click here to view our Standard Protocols