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TDP-43 Polyclonal antibody
TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, zebrafish and More (6)
Applications
FC, IF, IHC, WB, ELISA and More (5)
Conjugate
Unconjugated
1400
验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells |
| Positive IHC detected in | human gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Applications | FC, IF, IHC, WB, ELISA |
| Cited Applications | ChIP, CoIP, ELISA, IEM, IF, IHC, IP, RIP, WB |
| Tested Reactivity | human, mouse, rat, zebrafish |
| Cited Reactivity | human, mouse, rat, chicken, horse, Yeast, seal, monkey, zebrafish, hamster |
| Immunogen | fusion protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | TAR DNA binding protein |
| Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC001487 |
| Gene symbol | TARDBP |
| Uniprot | Q13148 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_615042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| IHC protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| IF protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lancet Neurol A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study. | ||
Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies | ||
