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  • KD/KO Validated

TDP-43 Polyclonal antibody

TDP-43 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish, kak and More (8)

Applications

FC, IF, IHC, WB, ELISA and More (5)

Conjugate

Unconjugated

Cat No : 10782-2-AP

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Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, K-562 cells, C2C12 cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, human brain tissue, human brain (FTLD-U) tissue, human gliomas tissue, human pancreas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, SH-SY5Y cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, kak samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications ChIP, CoIP, ELISA, IEM, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat, zebrafish, kak
Cited Reactivityhuman, mouse, Caenorhabditis elegans, canine, chicken, Drosophila, hamster, horse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
UniprotQ13148
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 antibody 10782-2-APDownload protocol
IHC protocol for TDP-43 antibody 10782-2-APDownload protocol
IF protocol for TDP-43 antibody 10782-2-APDownload protocol
FC protocol for TDP-43 antibody 10782-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Rep

Mutant TDP-43 Causes Early-Stage Dose-Dependent Motor Neuron Degeneration in a TARDBP Knockin Mouse Model of ALS.

Authors - Sarah Y Ebstein
humanIHC

Neurocase

Corticobasal syndrome with visual hallucinations and probable REM-sleep behavior disorder: an autopsied case report of a patient with CBD and LBD pathology.

Authors - George Naasan
humanWB,IF

Nat Neurosci

ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair.

Authors - Joseph R Klim
  • KD Validated
humanWB,IF

Neuron

Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death.

Authors - Fatima Gasset-Rosa
humanWB,IF

Nat Neurosci

Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration.

Authors - Ze'ev Melamed
human

Mol Genet Genomic Med

Autosomal dominant mitochondrial membrane protein-associated neurodegeneration (MPAN).

Authors - Allison Gregory