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TDP-43 (N-terminal) Polyclonal antibody

TDP-43 (N-terminal) Polyclonal Antibody for WB, IP, IF/ICC,IF-Fro, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (6)

Applications

WB, IP, IF/ICC,IF-Fro, IHC, ELISA and More (4)

Conjugate

Unconjugated

Cat No : 10782-2-AP

Print datasheet

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43



经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:3000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10782-2-AP targets TDP-43 (N-terminal) in WB, IP, IF, RIP, IHC, IEM, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications WB, IP, IF/ICC,IF-Fro, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, RIP, IHC, IEM, CoIP, ChIP, ELISA
Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, chicken, horse, Yeast, seal, monkey, zebrafish, hamster
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_615042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 (N-terminal) antibody 10782-2-APDownload protocol
IHC protocol for TDP-43 (N-terminal) antibody 10782-2-APDownload protocol
IF protocol for TDP-43 (N-terminal) antibody 10782-2-APDownload protocol
IP protocol for TDP-43 (N-terminal) antibody 10782-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
mouseIF

Cell

Mouse oocytes sequester aggregated proteins in degradative super-organelles

Authors - Gabriele Zaffagnini
humanWB

Lancet Neurol

A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.

Authors - Ilse Gijselinck
mouseIF

Cell Res

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Authors - Liang Guo
mouseIF

Nature

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Authors - Jiasheng Zhang
humanIF

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma