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验证数据展示

  • ChIP experiment of HeLa using 10782-2-AP

    ChIP experiment of HeLa using 10782-2-AP

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of TDP-43 Antibody(10782-2-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

  • IHC staining of human brain (FTLD-U) tissue using 10782-2-AP

    IHC staining of human brain (FTLD-U) tissue using 10782-2-AP

    40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis using 10782-2-AP

    WB analysis using 10782-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 10782-2-AP

    IHC staining of mouse brain using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human gliomas using 10782-2-AP

    IHC staining of human gliomas using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human gliomas using 10782-2-AP

    IHC staining of human gliomas using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of mouse brain using 10782-2-AP

    IHC staining of mouse brain using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of mouse brain using 10782-2-AP

    IHC staining of mouse brain using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis of HeLa using 10782-2-AP

    WB analysis of HeLa using 10782-2-AP

    WB result of TDP-43 antibody (10782-2-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

  • IHC staining of mouse brain using 10782-2-AP

    IHC staining of mouse brain using 10782-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IF Staining of mouse brain using 10782-2-AP

    IF Staining of mouse brain using 10782-2-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TDP-43 antibody (10782-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IP experiment of HeLa using 10782-2-AP

    IP experiment of HeLa using 10782-2-AP

    IP result of anti-TDP-43 (IP:10782-2-AP, 4ug; Detection:10782-2-AP 1:50000) with HeLa cells lysate 1520 ug.

  • IF Staining of HeLa using 10782-2-AP

    IF Staining of HeLa using 10782-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HeLa using 10782-2-AP

    FC experiment of HeLa using 10782-2-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (10782-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

TDP-43 Polyclonal antibody

TDP-43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA
Cat No. 10782-2-AP

产品说明书

引用文献(1558)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish and More (6)

应用

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA and More (4)

别名

TDP-43 (N-terminal), TDP 43, TARDBP, TDP43, TAR DNA-binding protein 43

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive ChIP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:3000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10782-2-AP targets TDP-43 in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA, IEM
经测试反应性 human, mouse, rat, zebrafish
文献引用反应性human, mouse, rat, monkey, chicken, zebrafish, hamster, yeast, horse, seal
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAR DNA binding protein
别名 TDP-43 (N-terminal), TDP 43, TARDBP, TDP43, TAR DNA-binding protein 43
计算分子量 43 kDa
观测分子量 44 kDa
GenBank蛋白编号BC001487
基因名称 TDP-43
Gene ID (NCBI) 23435
RRIDAB_615042
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13148
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

实验方案

Product Specific Protocols
WB protocol for TDP-43 antibody 10782-2-APDownload protocol
IHC protocol for TDP-43 antibody 10782-2-APDownload protocol
IF protocol for TDP-43 antibody 10782-2-APDownload protocol
IP protocol for TDP-43 antibody 10782-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
View Article
mouseIF

Cell

Mouse oocytes sequester aggregated proteins in degradative super-organelles

Authors - Gabriele Zaffagnini
View Article
humanWB

Lancet Neurol

A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.

Authors - Ilse Gijselinck
View Article
mouseIF

Cell Res

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Authors - Liang Guo
View Article
humanWB

Nature

MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.

Authors - Roberto Simone
View Article
humanIF

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma
View Article