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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Podocalyxin Polyclonal antibody

Podocalyxin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18150

Print datasheet

Synonyms

PODXL, PCLP 1, PCLP, PC, Gp200

验证数据展示

  • IF Staining of human kidney using CL488-18150

    IF Staining of human kidney using CL488-18150

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

  • IF Staining of human kidney using CL488-18150

    IF Staining of human kidney using CL488-18150

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy Podocalyxin Ready-To-Use IHC Kit. Podocalyxin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18150-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18150 targets Podocalyxin in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen Podocalyxin fusion protein Ag12844 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name podocalyxin-like
Synonyms PODXL, PCLP 1, PCLP, PC, Gp200
Calculated Molecular Weight 526 aa, 55 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC093730
Gene Symbol Podocalyxin
Gene ID (NCBI) 5420
RRIDAB_2919156
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00592
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It is Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in many cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Podocalyxin antibody CL488-18150Download protocol
Standard Protocols
Click here to view our Standard Protocols