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CoraLite® Plus 488-conjugated Podocalyxin Polyclonal antibody
Podocalyxin Polyclonal Antibody for FC (Intra), IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra), IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | human kidney tissue |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Podocalyxin Ready-To-Use IHC Kit. Podocalyxin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18150-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-18150 is Podocalyxin Fusion Protein expressed in E. coli.
| Tested Applications | FC (Intra), IF-P |
| Tested Reactivity | human |
| Immunogen | Podocalyxin fusion protein Ag12844 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | podocalyxin-like |
| Synonyms | GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60 |
| Calculated Molecular Weight | 526 aa, 55 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC093730 |
| Gene Symbol | PODXL |
| Gene ID (NCBI) | 5420 |
| RRID | AB_2919156 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00592 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Podocalyxin antibody CL488-18150 | Download protocol |
| FC protocol for CL Plus 488 Podocalyxin antibody CL488-18150 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
