验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain, rabbit brain, chicken brain |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary tumor tissue, human spleen tissue, human tonsillitis tissue, human skin cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66041-1-Ig targets HSP60 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, ELISA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, yeast |
| Immunogen | HSP60 fusion protein Ag7848 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Synonyms | 60 kDa chaperonin, Chaperonin 60, GROEL, Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC003030 |
| Gene Symbol | HSP60 |
| Gene ID (NCBI) | 3329 |
| RRID | AB_11041709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IHC protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IF protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| IP protocol for HSP60 antibody 66041-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Immunol Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. | ||
J Clin Invest ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage/cGAS-STING activation. | ||
Autophagy Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage. | ||
Autophagy Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival. |
