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  • Featured Product
  • KD/KO Validated

HSP60 Monoclonal antibody, PBS Only (Capture)

HSP60 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10E7

Cat no : 66041-1-PBS

Print datasheet

Synonyms

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

验证数据展示

  • WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

    WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

    IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPD1 antibody included
  • IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPD1 antibody included
  • IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

    IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

    IP result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

    IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1, HSPD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66041-1-PBS. Detection antibody: 66041-2-PBS. Standard:Ag7848. Range: 1.563-200 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.

产品信息

66041-1-PBS targets HSP60 as part of a matched antibody pair:

MP50644-1: 66041-1-PBS capture and 66041-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HSP60 fusion protein Ag7848 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 60kDa protein 1 (chaperonin)
Synonyms HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC003030
Gene Symbol HSP60
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)