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验证数据展示

  • WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

    WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

    IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPD1 antibody included
  • IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPD1 antibody included
  • IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

    IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

    IP result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

    IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1, HSPD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66041-1-PBS. Detection antibody: 66041-2-PBS. Standard:Ag7848. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

HSP60 Monoclonal antibody, PBS Only (Capture)

HSP60 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 66041-1-PBS

产品说明书

CloneNo. 2F10E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.

产品信息

66041-1-PBS targets HSP60 as part of a matched antibody pair:

MP50644-1: 66041-1-PBS capture and 66041-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HSP60 fusion protein Ag7848 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 60kDa protein 1 (chaperonin)
别名 HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60
计算分子量 61 kDa
观测分子量 60 kDa
GenBank蛋白编号BC003030
基因名称 HSP60
Gene ID (NCBI) 3329
RRIDAB_11041709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10809
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)