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验证数据展示

  • IF Staining of HepG2 using CL488-66041

    IF Staining of HepG2 using CL488-66041

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66041 (HSP60 antibody) at dilution of 1:50.

  • FC experiment of HepG2 using CL488-66041

    FC experiment of HepG2 using CL488-66041

    1X10^6 HepG2 cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human HSP60 (CL488-66041, Clone:2F10E7) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HSP60 Monoclonal antibody

HSP60 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66041

Print datasheet

CloneNo. 2F10E7
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra) and More (1)

Synonyms

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66041-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 1.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

RIPSee 1 publications below

产品信息

CL488-66041 targets HSP60 in IF/ICC, FC (Intra), RIP applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Cited ApplicationsRIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HSP60 fusion protein Ag7848 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 60kDa protein 1 (chaperonin)
Synonyms HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC003030
Gene Symbol HSP60
Gene ID (NCBI) 3329
RRIDAB_2883235
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10809
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HSP60 antibody CL488-66041Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Cancer Lett

circCCNY enhances lenvatinib sensitivity and suppresses immune evasion in hepatocellular carcinoma by serving as a scaffold for SMURF1 mediated HSP60 degradation

Authors - Lei Yang
View Article