FlexAble Biotin Antibody Labeling Kit for Rat IgG Kappa Light Chain
Application
IF, FC, IHC
Reactivity
Rat
Conjugate
Biotin
Cat No : KFA128
Validation Data Gallery
产品介绍
Product name | FlexAble Biotin Antibody Labeling Kit for Rat Kappa Light Chain |
Assay type | Antibody labeling |
Tested applications | IF, FC, IHC |
Species Reactivity | Rat Kappa Light Chain |
Antibody amount per labeling reaction | 0.5 µg antibody |
Conjugate | Biotin |
Compatible with | Streptavidin conjugated dyes and molecules |
| Component | 10 rxns | 50 rxns | 4×50 rxns |
Biotin FlexLinker for Rat Kappa Light Chain | 10 μL | 50 μL | 4×50 μL |
| FlexQuencher for Rat IgG Kappa Light Chain | 20 μL | 100 μL | 4×100 μL |
| FlexBuffer | 100 μL | 500 μL | 4×500 μL |
保存条件
Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.
其他
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.