验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue, hippocampus, human brain tissue, mouse cerebellum tissue, rat cerebellum tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue, human kidney tissue |
| Positive IF-Fro detected in | mouse kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14479-1-AP targets Calbindin-D28k in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 | Calbindin-D28k fusion protein Ag5861 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calbindin 1, 28kDa |
| 别名 | Calbindin, CALB1, D-28K, Calbindin D28, calbindin 1, 28kDa |
| 计算分子量 | 30 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC006478 |
| 基因名称 | Calbindin |
| Gene ID (NCBI) | 793 |
| RRID | AB_2228318 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05937 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IHC protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IF protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IP protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage | ||
Cell Death Differ The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum. | ||
Mol Ther sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model. |
