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  • Featured Product
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Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14479-1-AP

Print datasheet

Synonyms

Calbindin, CALB1, D-28K, Calbindin D28, calbindin 1, 28kDa

验证数据展示

  • IF Staining of mouse cerebellum tissue using 14479-1-AP

    IF Staining of mouse cerebellum tissue using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:1500 and Alexa Fluor 594 Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

  • WB analysis using 14479-1-AP

    WB analysis using 14479-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. (Negative control: NIH/3T3, C6)

  • IF Staining of mouse cerebellum using 14479-1-AP

    IF Staining of mouse cerebellum using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

  • IF Staining of mouse cerebellum using 14479-1-AP

    IF Staining of mouse cerebellum using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

  • IHC staining of human cerebellum using 14479-1-AP

    IHC staining of human cerebellum using 14479-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IHC staining of human cerebellum using 14479-1-AP

    IHC staining of human cerebellum using 14479-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IP experiment of mouse brain using 14479-1-AP

    IP experiment of mouse brain using 14479-1-AP

    IP result of anti-Calbindin-D28k (IP:14479-1-AP, 4ug; Detection:14479-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IF Staining of mouse cerebellum using 14479-1-AP

    IF Staining of mouse cerebellum using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human kidney using 14479-1-AP

    IF Staining of human kidney using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

  • IF Staining of mouse kidney using 14479-1-AP

    IF Staining of mouse kidney using 14479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, hippocampus, human brain tissue, mouse cerebellum tissue, rat cerebellum tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, human kidney tissue
Positive IF-Fro detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14479-1-AP targets Calbindin-D28k in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Immunogen Calbindin-D28k fusion protein Ag5861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calbindin 1, 28kDa
Synonyms Calbindin, CALB1, D-28K, Calbindin D28, calbindin 1, 28kDa
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
RRIDAB_2228318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

实验方案

Product Specific Protocols
WB protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IHC protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IF protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IP protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
View Article
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
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mouseIF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
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mouseIF

Cell Death Differ

The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.

Authors - Fei Wang
View Article
ratIF

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew
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mouseIHC,IF

Mol Ther

sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model.

Authors - Rui Zhang
View Article