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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14479

Print datasheet

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

验证数据展示

  • IF Staining of human kidney using CL594-14479

    IF Staining of human kidney using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

  • IF Staining of human kidney using CL594-14479

    IF Staining of human kidney using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

  • IF Staining of mouse cerebellum using CL594-14479

    IF Staining of mouse cerebellum using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman kidney tissue, mouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14479-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14479 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Calbindin-D28k fusion protein Ag5861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calbindin 1, 28kDa
Synonyms CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K
Calculated Molecular Weight 30 kDa
GenBank Accession NumberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
RRIDAB_2919807
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05937
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

实验方案

Product Specific Protocols
IF protocol for CL594 Calbindin-D28k antibody CL594-14479Download protocol
Standard Protocols
Click here to view our Standard Protocols