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验证数据展示

  • IF Staining of human kidney using CL594-14479

    IF Staining of human kidney using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

  • IF Staining of human kidney using CL594-14479

    IF Staining of human kidney using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

  • IF Staining of mouse cerebellum using CL594-14479

    IF Staining of mouse cerebellum using CL594-14479

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 Calbindin-D28k antibody (CL594-14479) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for IF-P
Cat No. CL594-14479

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman kidney tissue, mouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14479-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14479 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Calbindin-D28k fusion protein Ag5861 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calbindin 1, 28kDa
别名 CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K
计算分子量 30 kDa
GenBank蛋白编号BC006478
基因名称 Calbindin
Gene ID (NCBI) 793
RRIDAB_2919807
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05937
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

实验方案

Product Specific Protocols
IF protocol for CL594 Calbindin-D28k antibody CL594-14479Download protocol
Standard Protocols
Click here to view our Standard Protocols