Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for WB,IP,IHC,IF/ICC,IF-P,FC,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (6)





Cat No : 12987-1-AP

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ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1


Positive WB detected inHeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, human kidney tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human skin cancer tissue, NIH/3T3 cells
Positive FC detected inHEK-293 cells


Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


12987-1-AP targets Lamin B1 in WB, IP, IF, FC, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IP,IHC,IF/ICC,IF-P,FC,ELISA
Cited ApplicationsWB, IP, IF, FC, IHC, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, hamster, pig, Bombyx mori, canine, bovine
Immunogen Lamin B1 fusion protein Ag3631 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lamin B1
Synonyms ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC012295
Gene Symbol LMNB1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.


Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-APDownload protocol
IHC protocol for Lamin B1 antibody 12987-1-APDownload protocol
IF protocol for Lamin B1 antibody 12987-1-APDownload protocol
IP protocol for Lamin B1 antibody 12987-1-APDownload protocol
Standard Protocols
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