|Positive WB detected in
|HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
|Positive IP detected in
|Positive IHC detected in
|mouse heart tissue, human kidney tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, human skin cancer tissue, NIH/3T3 cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1000-1:4000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|FC, IF, IHC, IP, WB, ELISA
|ChIP, FC, IF, IHC, IP, WB
|human, mouse, rat
|human, mouse, rat, chicken, hamster, pig, Bombyx mori, canine, bovine
|Lamin B1 fusion protein Ag3631 种属同源性预测
|Host / Isotype
|Rabbit / IgG
|ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
|Product Specific Protocols
|WB protocol for Lamin B1 antibody 12987-1-AP
|IHC protocol for Lamin B1 antibody 12987-1-AP
|IF protocol for Lamin B1 antibody 12987-1-AP
|IP protocol for Lamin B1 antibody 12987-1-AP
|Click here to view our Standard Protocols
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies
Chromothripsis drives the evolution of gene amplification in cancer.
Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.
The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.
Cell Host Microbe
Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice