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Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12987-1-AP

Print datasheet

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

验证数据展示

  • WB analysis using 12987-1-AP

    WB analysis using 12987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12987-1-AP

    IF Staining of HepG2 using 12987-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 12987-1-AP

    IHC staining of human liver cancer using 12987-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using 12987-1-AP

    FC experiment of HEK-293 using 12987-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug Lamin B1 antibody (12987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

  • IP experiment of HeLa using 12987-1-AP

    IP experiment of HeLa using 12987-1-AP

    IP Result of anti-LMNB1 (IP:12987-1-AP, 3ug; Detection:12987-1-AP 1:1000) with HeLa cells lysate 2500ug.

  • WB analysis using 12987-1-AP

    WB analysis using 12987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12987-1-AP

    IF Staining of HepG2 using 12987-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human skin cancer tissue, NIH/3T3 cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications ChIP, FC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Bombyx mori, canine, chicken, hamster, pig
Immunogen Lamin B1 fusion protein Ag3631 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lamin B1
Synonyms ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene symbol LMNB1
UniprotP20700
Gene ID (NCBI) 4001
RRIDAB_2136290
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

实验方案

Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-APDownload protocol
IHC protocol for Lamin B1 antibody 12987-1-APDownload protocol
IF protocol for Lamin B1 antibody 12987-1-APDownload protocol
IP protocol for Lamin B1 antibody 12987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Biotechnol

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

Authors - Matthew T N Yarnall
View Article
humanIF

Nature

Chromothripsis drives the evolution of gene amplification in cancer.

Authors - Ofer Shoshani
View Article
WB

Nature

Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.

Authors - Yingfeng Li
View Article
humanWB

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
View Article
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
View Article
humanWB

Cancer Discov

Posttranslational regulation of the exon skipping machinery controls aberrant splicing in leukemia.

Authors - Yalu Zhou
View Article