GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, pig, arabidopsis , corn , cabbage, rice and More (8)


FC, IF, IHC, IP, WB, ELISA and More (3)



Cat No : 10494-1-AP

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Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.


Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery


10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications Cell treatment, CoIP, ELISA, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat, pig, arabidopsis , corn , cabbage, rice
Cited Reactivityhuman, rat, goat, treeshrew, Chicken, Yeast, leech, Arabidopsis, zebra finches, sheep
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.


Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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