GAPDH Polyclonal antibody
GAPDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig, arabidopsis, cabbage, corn, rice and More (7)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (5)
Conjugate
Unconjugated
7018
验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:40000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10494-1-AP targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig, arabidopsis, cabbage, corn, rice samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA, Cell treatment |
Tested Reactivity | human, mouse, rat, pig, arabidopsis, cabbage, corn, rice |
Cited Reactivity | human, rat, chicken, goat, sheep, yeast, leech, treeshrew, arabidopsis, zebra finches |
Immunogen | GAPDH fusion protein Ag0766 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glyceraldehyde-3-phosphate dehydrogenase |
Synonyms | |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC004109 |
Gene Symbol | GAPDH |
Gene ID (NCBI) | 2597 |
RRID | AB_2263076 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04406 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GAPDH antibody 10494-1-AP | Download protocol |
IHC protocol for GAPDH antibody 10494-1-AP | Download protocol |
IF protocol for GAPDH antibody 10494-1-AP | Download protocol |
IP protocol for GAPDH antibody 10494-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival | ||
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||