GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, arabidopsis , corn , cabbage, rice and More (9)

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10494-1-AP

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



经过测试的应用

Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

产品信息

10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat, pig, arabidopsis , corn , cabbage, rice
Cited Reactivityhuman, Arabidopsis, Bovine, Caenorhabditis elegans, canine, chicken, EPC cells, fish, goat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
UniprotP04406
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
FC protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Med

Carbon monoxide-releasing molecule suppresses inflammatory and osteoclastogenic cytokines in nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via the heme oxygenase-1 pathway.

Authors - Ling Song
humanWB

Bosn J Basic Med Sci

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).

Authors - Peng Li
humanWB

BMC Pregnancy Childbirth

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.

Authors - Xiaotong Sun
humanWB

Cancer Sci

PMID: 30417588

Authors - Chao Qu
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
humanWB

Int J Mol Med

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Authors - Wei Li